Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ZCL278 is a selective Cdc42 GTPase inhibitor.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 46.00 | |
10 mg | In stock | $ 72.00 | |
25 mg | In stock | $ 138.00 | |
50 mg | In stock | $ 233.00 | |
100 mg | In stock | $ 327.00 | |
200 mg | In stock | $ 523.00 | |
500 mg | In stock | $ 848.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 72.00 |
Description | ZCL278 is a selective Cdc42 GTPase inhibitor. |
Targets&IC50 | GTPase CDC42:11.4 μM(Kd) |
In vivo | ZCL278 reduces the JUNV RNA load in the spleen more than 33-fold, with JUNV RNA being undetectable in 5 out of 8 mice. These results are similar to those seen in Gabapentin-treated mice, indicating that ZCL278 is able to abrogate JUNV replication[2]. |
Kinase Assay | Lyophilized Cdc42 protein is reconstituted to 5 mg/mL in a buffer consisting of 50 mM Tris, 0.5 mM MgCl2, 50 mM NaCl, 3% (wt/vol) sucrose, and 0.6% dextran. The stock solution is then diluted to 1 μM in 5 mM phosphate buffer, pH 7.4. Into a quartz cuvettete containing Cdc42 solution, aliquots of ZCL278 are added and incubated for 5 min before each fluorescent measurement. The excitation wavelength is 275 nm, and the fluorescence of tryptophan at 350 nm is measured after each addition. The titration curve is fitted using the equimolar specific binding model in GraphPad, and the Kd is calculated[1]. |
Cell Research | ZCL278 is prepared in DMSO and stored, and then diluted with appropriate medium before use[1]. To determine cell viability, PC-3 cells are incubated for 24 h with or without the Cdc42 activator, ZCL278, or NSC23766. By using the trypan blue dye exclusion method, the numbers of live and dead cells are obtained with a Countess Automated Cell Counter. P values are assigned in each experiment, and any null hypothesis with probability level <95% is rejected[1]. |
Synonyms | ZCL 278 |
Molecular Weight | 584.89 |
Formula | C21H19BrClN5O4S2 |
CAS No. | 587841-73-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 58.5 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
ZCL278 587841-73-4 Cell Cycle/Checkpoint GPCR/G Protein MAPK CDK Ras organization Golgi prostate actin-based cellular Inhibitor motility ZCL 278 metastatic inhibit Cdc42 Cdc42-ZCL278 ZCL-278 cancer inhibitor