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YS-49

Catalog No. T13376 CAS 132836-42-1

YS-49 is an activator of PI3K/Akt (a downstream target of RhoA).

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YS-49, CAS 132836-42-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	YS-49 is an activator of PI3K/Akt (a downstream target of RhoA).
In vitro	In RAVSMC and RAW 264.7 cells, YS-49 (1-100?μM; 18 hours; ) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC50 values of 22 μM and 30?μM, respectively[2].At the transcriptional level, YS-49 (10-100?μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells [2].
In vivo	In male Sprague Dawley rats,YS-49 (5?mg/kg; intraperitoneal injection; 8 hours) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM[2].

Molecular Weight	386.28
Formula	C20H20BrNO2
CAS No.	132836-42-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 10 mg/mL (25.89 mM）, ultrasonic and warming and heat to 60°C

DMSO: 100 mg/mL (258.88 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Sun JJ, Kim HJ, Seo HG, et al. YS49,1-(alpha-naphtylmethyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,regulates angiotensin II-stimulated ROS production, JNK phosphorylation and vascular smooth muscle cell proliferation via the induction of heme oxygenase-1. Life Sci. 2008;82(11-12):600-7. 2. Kang YJ, et al. Prevention of the expression of inducible nitric oxide synthase by a novel positive inotropic agent, YS 49, in rat vascular smooth muscle and RAW 264.7 macrophages. Br J Pharmacol. 1999 Sep;128(2):357-64. 3. Hsu YH, et al. RhoA-mediated inhibition of vascular endothelial cell mobility: positive feedback through reduced cytosolic p21 and p27. J Cell Physiol. 2014 Oct;229(10):1455-65.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

YS-49 132836-42-1 Cytoskeletal Signaling Endocrinology/Hormones GPCR/G Protein Neuroscience PI3K/Akt/mTOR signaling Adrenergic Receptor RAAS PI3K Akt inhibit Phosphoinositide 3-kinase PKB Beta Receptor YS 49 Protein kinase B Inhibitor YS49 Angiotensin Receptor inhibitor

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