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Topotecan hydrochloride

Catalog No. T1174 CAS 119413-54-6

Synonyms: SKF 104864A, SKFS 104864A, NSC609699, Nogitecan HCl, NSC 609669, Topotecan HCl

Topotecan hydrochloride (NSC609699) is an antineoplastic agent used to treat ovarian cancer.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Topotecan hydrochloride Chemical Structure

Topotecan hydrochloride, CAS 119413-54-6

Pack Size	Availability	Price/USD
5 mg	In stock	$ 30.00
10 mg	In stock	$ 47.00
25 mg	In stock	$ 73.00
50 mg	In stock	$ 109.00
100 mg	In stock	$ 147.00
500 mg	In stock	$ 363.00
1 g	In stock	$ 538.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 98.76%

Purity: 98.71%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Topotecan hydrochloride (NSC609699) is an antineoplastic agent used to treat ovarian cancer.
Targets&IC50	Topo I (DU-145 Luc cells):2 nM, Topo I (MCF-7 Luc cells):13 nM
In vitro	Topotecan induces effective anti-leukemic activity in a severe combined immunodeficient (SCID) mouse model of poor-prognosis acute lymphoblastic leukemia (ALL). Its administration significantly prolongs the disease-free survival in SCID mice challenged with a lethal dose of human leukemia cells at systemic drug exposure levels. Glioblastomas preferentially express TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), and Topotecan treatment notably upregulates this expression. Furthermore, when DU-145 Luc cells are subcutaneously implanted and treated with Topotecan, significant tumor growth inhibition and regression are observed via caudal vein injection and bioluminescent imaging.
In vivo	In radiation-resistant human B-lineage acute lymphoblastic leukemia cells, Topotecan stabilizes topoisomerase I/DNA cleavable complexes, inducing rapid apoptosis regardless of high bcl-2 protein expression, and inhibits clonal growth of the cells in a dose-dependent manner. Topotecan exhibits augmented drug activity against DU-145 Luc and MCF-7 Luc cells. During DNA replication, its cytotoxic action is mediated by stabilizing the covalent complex between topoisomerase I and DNA, preventing religation of the enzyme-linked single-strand DNA breaks.
Cell Research	Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager. (Only for Reference)
Source	Natural Products > Nyssaceae > Camptotheca

Synonyms	SKF 104864A, SKFS 104864A, NSC609699, Nogitecan HCl, NSC 609669, Topotecan HCl
Molecular Weight	457.92
Formula	C23H24ClN3O5
CAS No.	119413-54-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 45.8 mg/mL (100 mM)

DMSO: 45.8 mg/mL (100 mM)

References and Literature

1. Caceres G, et al. Anticancer Drugs. 2003, 14(7), 569-574. 2. Uckun FM, et al. Blood. 1995. 85(10), 2817-2828. 3. Ciusani E, et al. J Neurooncol. 2005, 71(1), 19-25. 4. McCluskey AG, et al. J Nucl Med. 2012, 53(7), 1146-1154. 5. Romanelli S, et al. Cancer Chemother Pharmacol. 1998, 41(5), 385-390.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library EMA Approved Drug Library Anti-Cancer Active Compound Library Anti-Cancer Approved Drug Library Drug Repurposing Compound Library Bioactive Compound Library FDA-Approved & Pharmacopeia Drug Library Chemotherapy Drug Library

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Related compounds with same targets

Chetomin Fludarabine 2-methoxycinnamaldehyde MI-3 PRIMA-1 Ginsenoside Rh2 dBET6 SAR125844

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Keywords

Topotecan hydrochloride 119413-54-6 Apoptosis Autophagy DNA Damage/DNA Repair Topoisomerase SKF 104864A NSC609669 SKFS 104864A NSC609699 NSC-609699 Inhibitor Nogitecan HCl NSC 609669 NSC 609699 NSC-609669 Topotecan Hydrochloride inhibit Topotecan HCl Topotecan inhibitor

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