Powder: -20°C for 3 years
In solvent: -80°C for 2 years
Pelargonidin chloride shows protective effect against CTN-induced oxidative stress in HepG2 cells and up-regulated the activity of detoxification enzyme levels through Keap1/Nrf2 signaling pathway.
Description | Pelargonidin chloride shows protective effect against CTN-induced oxidative stress in HepG2 cells and up-regulated the activity of detoxification enzyme levels through Keap1/Nrf2 signaling pathway. |
In vitro | The effect of PC in ameliorating citrinin (CTN) induced cytotoxicity and oxidative stress. The cytotoxicity of CTN was evaluated by treating HepG2 (Human hepatocellular carcinoma) cells with CTN (0-150 μM) in a dose dependent manner for 24 h, and the IC50 was determined to be 96.16 μM. CTN increased lactate dehydrogenase leakage (59%), elevated reactive oxygen species (2.5-fold), depolarized mitochondrial membrane potential as confirmed by JC-1 monomers and arrested cell cycle at G2/M phase. Further, apoptotic and necrotic analysis revealed significant changes followed by DNA damage. To overcome these toxicological effects, PC was pretreated for 2 h followed by CTN exposure for 24 h. Pretreatment with PC resulted in significant increase in cell viability (84.5%), restored membrane integrity, reactive oxygen species level were maintained and cell cycle phases were normal. PC significantly up-regulated the activity of detoxification enzymes: heme oxygenase 1 (HO-1), glutathione transferase, glutathione peroxidase, superoxide dismutase and quinone reductase. Nrf2 translocation into the nucleus was also observed by immunocytochemistry analysis[1] |
Molecular Weight | 306.7 |
Formula | C15H11ClO5 |
CAS No. | 134-04-3 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 10 mM
( < 1 mg/ml refers to the product slightly soluble or insoluble )
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Pelargonidin chloride 134-04-3 NF-Kb信号通路 代谢 免疫与炎症 NO Synthase NOS Nrf2 Reactive Oxygen Species Inhibitor Pelargonidin Radical antioxidant scavenger inhibit nitric oxide inhibitor