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PR-619

Catalog No. T1862 CAS 2645-32-1

Synonyms: PR 619, 2,6-Diamino-3,5-dithiocyanopyridine

PR-619 (2,6-Diamino-3,5-dithiocyanopyridine) is a non-selective, reversible inhibitor of the deubiquitinylating enzymes (DUBs).

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PR-619, CAS 2645-32-1

Pack Size	Availability	Price/USD
5 mg	In stock	$ 45.00
10 mg	In stock	$ 55.00
25 mg	In stock	$ 83.00
50 mg	In stock	$ 113.00
100 mg	In stock	$ 158.00
200 mg	In stock	$ 238.00
500 mg	In stock	$ 393.00
1 mL * 10 mM (in DMSO)	In stock	$ 79.00

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Purity: 100%

Purity: 99.83%

Purity: 99.10%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	PR-619 (2,6-Diamino-3,5-dithiocyanopyridine) is a non-selective, reversible inhibitor of the deubiquitinylating enzymes (DUBs).
Targets&IC50	USP4:3.93 μM(EC50), JOSD2:1.17 μM(EC50), UCHL3:2.95 μM(EC50), SENP6 core:2.37 μM(EC50), USP8:4.90 μM(EC50)
Kinase Assay	Ub-PLA2 assay: Recombinant enzymes in 20 mM Tris-HCl, pH 8.0, 2 mM CaCl2 and 2 mM β-mercaptoethanol (DUB assay buffer) are preincubated with single doses or dose ranges of PR-619 or P22077 for 30 minutes in a 96 well plate before the addition of Ub-PLA2 and NBD C6-HPC. The liberation of a fluorescent product within the linear range of the assay is monitored at room temperature using a fluorescence plate reader. Vehicle (2%(v/v) DMSO) and 10 mM N-ethylmaleimide are included as controls. Where ≥60% inhibition is observed, EC50 values are determined using a sigmoidal dose response equation.
Cell Research	72 h hours later, 0.2 mg/mL resazurin prepared in phosphate-buffered saline is added to each well and the cells are incubated for an additional 3-6 h. The fluorescence of the resazurin reduction product is measured using Ex=535 nm and Em=590 nm filters on a fluorimeter. The EC50 values are calculated in Prism.(Only for Reference)

Synonyms	PR 619, 2,6-Diamino-3,5-dithiocyanopyridine
Molecular Weight	223.28
Formula	C7H5N5S2
CAS No.	2645-32-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 201.5 mM

References and Literature

1. Altun M, et al. Chem Biol, 2011, 18(11), 1401-1412. 2. Song P, Yang S, Hua H, et al. The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells[J]. Journal of Biological Chemistry, 2019: jbc. RA118. 006029.

Citations

1. Song P, Yang S, Hua H, et al. The regulatory protein GADD34 inhibits TRAIL-induced apoptosis via TRAF6/ERK-dependent stabilization of myeloid cell leukemia 1 in liver cancer cells. Journal of Biological Chemistry. 2019: jbc. RA118. 006029

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library NO PAINS Compound Library Bioactive Compound Library Anti-Cancer Compound Library Cell Cycle Compound Library Bioactive Compounds Library Max HIF-1 Signaling Pathway Compound Library Apoptosis Compound Library Anti-Aging Compound Library Endoplasmic Reticulum Stress Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

PR-619 2645-32-1 Apoptosis Autophagy Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB DUBs PR619 tau G0/G1 Deubiquitinase PR 619 ER-stress 2,6-Diamino-3,5-dithiocyanopyridine ubiquitination inhibit Inhibitor arrest inhibitor

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