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Oligomycin A

Name: Oligomycin A
Brand: TargetMol
SKU: T6323
Price: 50 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6323Cas No. 579-13-5

Alias MCH 32

Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

Oligomycin A

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Purity: 99.99%

Catalog No. T6323Alias MCH 32Cas No. 579-13-5

Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$50	In Stock	In Stock
2 mg	$65	In Stock	In Stock
5 mg	$123	In Stock	In Stock
10 mg	$197	In Stock	In Stock
25 mg	$396	In Stock	In Stock
50 mg	$635	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$135	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.99%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.
Targets&IC50	SH-SY5Y cells:29 μM, HCT116 cells:1 μM, HCT116 cells:0.9 μM, K562 cells:0.2 μM, F0F1-ATPase:1 μM (Ki)
In vitro	METHODS: Human melanoma cells WM3734 were treated with Oligomycin A (0.01-1 µg/mL) for 24 h, and lactate production was measured in culture supernatants. RESULTS: Oligomycin A caused an increase in lactate production in WM3734 cells. [1] METHODS: Human lung cancer cells H1299 were treated with Oligomycin A (0.1-1000 ng/mL) for 100 min, and cellular respiration was measured using Oxygen Biosensor System plates. RESULTS: At 100 and 1000 ng/mL, Oligomycin A completely inhibited cellular respiration within approximately one hour. [2]
In vivo	METHODS: To assay anti-inflammatory activity, Oligomycin A (0.25 mg/kg) was injected intraperitoneally into C57BL6 mice in a psoriasis model once daily for five days. RESULTS: Oligomycin A reduced ear thickness, keratinocyte proliferation and immune cell infiltration. [3]
Cell Research	ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference)
Synonyms	MCH 32

Chemical Properties

Molecular Weight	791.06
Formula	C₄₅H₇₄O₁₁
Cas No.	579-13-5
Smiles	[H][C@]12CC[C@@H](CC)\C=C\C=C\C[C@@H](C)[C@H](O)[C@@](C)(O)C(=O)[C@@H](C)[C@H](O)[C@@H](C)C(=O)[C@@H](C)[C@H](O)[C@@H](C)\C=C\C(=O)O[C@]([H])([C@@H]1C)[C@H](C)[C@@]1(CC[C@H](C)[C@H](C[C@@H](C)O)O1)O2
Relative Density.	1.14g/cm3

Storage & Solubility Information

Storage

store at low temperature,keep away from moisture | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 10 mg/mL (12.64 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (1.26 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.2641 mL	6.3206 mL	12.6413 mL	63.2063 mL
5 mM	0.2528 mL	1.2641 mL	2.5283 mL	12.6413 mL
10 mM	0.1264 mL	0.6321 mL	1.2641 mL	6.3206 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc