store at low temperature,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Nutlin-3a ((−)-Nutlin-3), the active enantiomer of Nutlin-3, inhibits MDM2-p53 interactions and stabilizes the p53 protein.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 45.00 | |
2 mg | In stock | $ 64.00 | |
5 mg | In stock | $ 97.00 | |
10 mg | In stock | $ 167.00 | |
25 mg | In stock | $ 282.00 | |
50 mg | In stock | $ 418.00 | |
100 mg | In stock | $ 618.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 137.00 |
Description | Nutlin-3a ((−)-Nutlin-3), the active enantiomer of Nutlin-3, inhibits MDM2-p53 interactions and stabilizes the p53 protein. |
Targets&IC50 | MDM2-p53 interaction:90 nM |
Kinase Assay | Biacore studies: Competition assays are performed on a Biacore S51. A Series S Sensor chip CM5 is derivatized for immobilization of a PentaHis antibody for capture of the His-tagged p53. The level of capture is ~ 200 response units (1 response unit corresponds to 1 pg of protein per mm 2). The concentration of MDM2 protein is kept constant at 300 nM. Test compounds are dissolved in DMSO at 10 mM and further diluted to make a concentration series of inhibitor in each MDM2 test sample. The assays are run at 25 °C in running buffer (10 mM Hepes, 0.15 M NaCl, 2% DMSO). MDM2-p53 binding in the presence of inhibitor is calculated as a percentage of binding in the absence of inhibitor and IC50 is calculated using Microsoft Excel |
Cell Research | SRB(Only for Reference) |
Synonyms | (-)-Nutlin-3, Nutlin-3a chiral, (−)-Nutlin-3 |
Molecular Weight | 581.49 |
Formula | C30H30Cl2N4O4 |
CAS No. | 675576-98-4 |
store at low temperature,keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 58.2 mg/mL (100 mM)
DMSO: 55 mg/mL (94.58 mM)
5% DMSO+95% Saline: 2.91 mg/mL (5 mM, suspension)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Nutlin-3a 675576-98-4 Apoptosis Autophagy Ubiquitination E1/E2/E3 Enzyme E1 activating enzyme inhibit E3 ligating enzyme p53 carcinomas ovarian (-)-Nutlin-3 Nutlin-3 Ubiquitin ligase Ubiquitin conjugating enzyme Ubiquitin activating enzyme Nutlin 3a Inhibitor Nutlin3a Nutlin-3a chiral MDM-2/p53 (−)-Nutlin-3 Rebemadlin E2 conjugating enzyme inhibitor