Home Tools
Log in
Cart

Nocodazole

Catalog No. T2802   CAS 31430-18-9
Synonyms: R17934, Oncodazole

Nocodazole (Oncodazole) is a reversible inhibitor of microtubule polymerization and an inhibitor of Bcr-Abl. Nocodazole has antitumor activity, blocks the cell cycle and induces apoptosis.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Nocodazole Chemical Structure
Nocodazole, CAS 31430-18-9
Pack Size Availability Price/USD Quantity
5 mg In stock $ 31.00
10 mg In stock $ 50.00
25 mg In stock $ 100.00
50 mg In stock $ 177.00
100 mg In stock $ 297.00
200 mg In stock $ 443.00
500 mg In stock $ 718.00
1 mL * 10 mM (in DMSO) In stock $ 34.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 99.91%
Purity: 99.34%
Purity: 98%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description Nocodazole (Oncodazole) is a reversible inhibitor of microtubule polymerization and an inhibitor of Bcr-Abl. Nocodazole has antitumor activity, blocks the cell cycle and induces apoptosis.
Targets&IC50 Abl (E255K):0.53 μM, Abl:0.21 μM, Abl (T315I):0.64 μM
In vitro In the COLO205 tumor xenograft model, the synergistic use of Nocodazole with Ketoconazole enhances apoptosis induction and exerts a stronger inhibitory effect on tumor volume and weight in mice.
In vivo Nocodazole demonstrates high affinity for oncogenic kinases, such as phosphorylated ABL (Kd: 0.091 μM), c-KIT (Kd: 1.6 μM), BRAF (Kd: 1.8 μM), and MEK (Kd: 1.6 μM). It also exhibits significant affinity towards ABL(E255K) phosphorylation (Kd: 0.12 μM), ABL(T315I) phosphorylation (Kd: 0.17 μM), BRAF(V600E) (Kd: 1.1 μM), and PI3Kγ (Kd: 1.5 μM). At high concentrations, Nocodazole can rapidly depolymerize microtubules in cells, whereas at lower concentrations, it inhibits the dynamic instability of microtubules. Treatment with Nocodazole for 6 hours to synchronize cells in the mitotic phase, followed by the addition of varying concentrations of Taxol, results in G1 phase arrest (IC50: 4 nM).
Cell Research Nocodazole is dissolved in a final concentration of 0.05% DMSO. Proteins are loaded at 50 μg/lane and separated by 12% (w:v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and probed with antibodies for cyclin E, p53, p21/CIP1, p27/KIP1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclin A, cyclin D1, cyclin D3, cyclin B, CDK2, CDK4, and cytochrome C. Immunoreactive bands are visualized by incubating with the colorigenic substrates nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate. The expression of GAPDH is used as the control for equal protein loading.
Synonyms R17934, Oncodazole
Molecular Weight 301.32
Formula C14H11N3O3S
CAS No. 31430-18-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 12.5 mg/mL (41.48 mM)

TargetMolReferences and Literature

1. Signoretto E, et al. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2016;38(1):379-92. 2. Yiangou L, et al. Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics. Stem Cell Reports. 2019 Jan 8;12(1):165-179. 3. Feng R, et al. Targeting the microtubular network as a new antimyeloma strategy. Mol Cancer Ther. 2011 Oct;10(10):1886-96. 4. Wang YJ, et al. Ketoconazole potentiates the antitumor effects of nocodazole: In vivo therapy for human tumor xenografts in nude mice. Mol Carcinog. 2002 Aug;34(4):199-210. 5. Wang YJ, et al. Mol Carcinog. 2002, 34(4), 199-210. 6. Signoretto E, et al. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2016;38(1):379-92. 7. Zhang JP, et al. Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage. Genome Biol. 2017 Feb 20;18(1):35. 8. Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules[J]. Macromolecular bioscience. 2019: 1900105. 9. Reyes G X, Kolodziejczak A, Devakumar L J P S, et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair[J]. Current Biology. 10. Bai Y, Wang W, Li S, et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex[J]. Molecular Cell. 2019.

TargetMolCitations

1. Bai Y, Wang W, Li S, et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. Molecular Cell. 2019 2. Moreira G G, Cantrelle F X, Quezada A, et al. Dynamic interactions and Ca2+-binding modulate the holdase-type chaperone activity of S100B preventing tau aggregation and seeding. Nature Communications. 2021, 12(1): 1-16 3. Reyes G X, Kolodziejczak A, Devakumar L J P S, et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair. Current Biology.. 2021 Mar 22;31(6):1268-1276.e6. doi: 10.1016/j.cub.2020.12.018. Epub 2021 Jan 7. 4. Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules. Macromolecular bioscience. 2019: 1900105. 5. Cui L, Xu Q, Zhang P, et al.Biochemical reprogramming of active-targeting and redox responsive nanoparticles for enhancing selective internalization.Materials Letters.2023: 134379. 6. Wang J, Chen Y, Li S, et al.PP2A inhibition causes synthetic lethality in BRCA2-mutated prostate cancer models via reactivating spindle assembly checkpoint.The Journal of Clinical Investigation.2023

Related compound libraries

This product is contained In the following compound libraries:
Microtubule-Targeted Compound Library Tyrosine Kinase Inhibitor Library Anti-Neurodegenerative Disease Compound Library Anti-Cancer Active Compound Library Autophagy Compound Library Human Metabolite Library Angiogenesis related Compound Library Anti-Cancer Metabolism Compound Library Hematopoietic Toxicity Compound Library Cytoskeletal Signaling Pathway Compound Library

Related Products

Related compounds with same targets
Dasatinib PD180970 AN-019 SIAIS100 TFA BGG463 Pivanex Nilotinib hydrochloride PP121

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Nocodazole 31430-18-9 Angiogenesis Apoptosis Autophagy Cytoskeletal Signaling DNA Damage/DNA Repair Tyrosine Kinase/Adaptors Microtubule Associated Bcr-Abl CRISPR/Cas9 Inhibitor R17934 Microtubule/Tubulin Oncodazole inhibit R 17934 R-17934 inhibitor

 

TargetMol