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XI-006

Catalog No. T6610 CAS 58131-57-0

Synonyms: NSC 207895

XI-006 (XI-006) suppresses MDMX with IC50 of 2.5 μM, leading to enhanced p53 stabilization/activation and DNA damage, and also regulates MDM2, an E3 ligase.

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XI-006, CAS 58131-57-0

Pack Size	Availability	Price/USD
1 mg	In stock	$ 51.00
2 mg	In stock	$ 74.00
5 mg	In stock	$ 122.00
10 mg	In stock	$ 197.00
25 mg	In stock	$ 455.00
50 mg	In stock	$ 672.00
100 mg	In stock	$ 956.00
500 mg	In stock	$ 1,920.00
1 mL * 10 mM (in DMSO)	In stock	$ 116.00

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Purity: 99.12%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	XI-006 (XI-006) suppresses MDMX with IC50 of 2.5 μM, leading to enhanced p53 stabilization/activation and DNA damage, and also regulates MDM2, an E3 ligase.
Targets&IC50	MDMX:2.5 μM
In vitro	XI-006 decreases both the MDMX mRNA and protein in MCF-7 cells. XI-006 induces expression of p53 as well as well-characterized p53-target gene, p21 and MDM2, in a dose-dependent manner in MCF-7 cells. XI-006 extends the half-life of p53 from 20 to 30 minutes to more than 3 hours as revealed by cycloheximide chase assays in MCF-7 cells. XI-006 also activates p53 and induces p21 and MDM2 expression in LNCaP prostate and A549 lung cancer cells. XI-006 increases the mRNA levels of proapoptotic genes including PUMA, BAX, and PIG3 in a dose-dependent manner in MCF-7 cells. XI-006 results in a significant increase in the numbers of sub-G0/G1 cells as well as G2 arrest. XI-006 also results in more than 40% of cells dying via apoptosis and decreases cell viability in A549 and LNCaP cells. [1] XI-006 inhibits biosynthesis of nucleic acids and proteins in L1210 cells. [2] XI-006 interacts with DNA repair to activate the DNA damage repair pathway in three species (S. cerevisiae, S. pombe and H. sapiens). [3] XI-006 acts as cytotoxic agent in the G/R-luc astrocytoma cell line with GI50 of 117 nM. [4]
Cell Research	MCF-7 cells treated with dimethyl sulfoxide (DMSO), nutlin-3a, or NSC-207895 are permeabilized with cold 70% ethanol overnight, and stained with a solution containing 50 μg/mL propidium iodide and 20 μg/mL RNase A at 37°C for 20 minutes. The cells are then subjected to flow cytometry analysis. The FlowJo software is used to calculate percentages of cells in each cell cycle phase. For terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL) staining, MCF-7 cells treated with the NSC-207895 for 2 days are fixed with 4% paraformadelhyde for 1 hour, and then subjected to dUTP labeling using In Situ Cell Death Detection Kit TMR Red according to the manufacturer's protocol. For quantitation, at least 300 cells are randomly chosen and the numbers of TUNEL-positive cells are counted. (Only for Reference)

Synonyms	NSC 207895
Molecular Weight	279.25
Formula	C11H13N5O4
CAS No.	58131-57-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 55 mg/ml (196.96 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Wang H, et al. Mol Cancer Ther, 2011, 10(1), 69-79. 2. Kessel D, et al. Cancer Res, 1975, 35(12), 3735-3740. 3. Kapitzky L, et al. Mol Syst Biol, 2010, 6, 451. 4. Hawes JJ, et al. J Biomol Screen, 2008, 13(8), 795-803.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Colorectal Cancer Compound Library Transcription Factor-Targeted Compound Library Apoptosis Compound Library Anti-Cancer Metabolism Compound Library Anti-Aging Compound Library Bioactive Compounds Library Max PPI Inhibitor Library Anti-Pancreatic Cancer Compound Library Ubiquitination Compound Library

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SP-141 Kevetrin hydrochloride SJ-172550 MD-224 IVHD-valtrate MI-1061 NSC 66811 MMRi64

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

XI-006 58131-57-0 Apoptosis Mdm2 p53 LPS MDMX XI006 Inhibitor NSC-207895 inhibit MDM2 XI 006 MDM-2/p53 NSC 207895 NSC207895 inhibitor

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