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Doxycycline (hyclate)

Catalog No. T1687L   CAS 24390-14-5
Synonyms: Doxycycline hydrochloride hemiethanolate hemihydrate, WC2031, Doxycycline hyclate

Doxycycline hyclate (WC2031) is a synthetic tetracycline derivative with similar antimicrobial activity. It is also an inhibitor of matrix metalloproteinases (MMP).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Doxycycline (hyclate) Chemical Structure
Doxycycline (hyclate), CAS 24390-14-5
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100 mg In stock $ 41.00
1 g In stock $ 48.00
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Purity: 100%
Purity: 99.94%
Purity: 99.75%
Purity: 99.75%
Purity: 99.17%
Purity: 98.76%
Purity: 98.76%
Purity: 98.66%
Purity: 98.55%
Purity: 97.53%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Doxycycline hyclate (WC2031) is a synthetic tetracycline derivative with similar antimicrobial activity. It is also an inhibitor of matrix metalloproteinases (MMP).
Kinase Assay Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size.
Cell Research Doxycycline is diluted in culture medium at a concentration of 10 μg/mL. All in vitro treatments are performed in SMC cultures at 90% confluence, when ECM synthesis in SMC starts to be evident. Doxycycline (20 nM) is diluted in culture medium at a concentration of 10 μg/mL (20 nM), at which no toxicity or variation in primary cultured SMC proliferation has been reported, as well as in other cell lines and the incubation time is 48 h. SMC-C and SMC-Ch are seeded at equal cell density in 6-well plates and, when confluence reaches 90%, 1 mL culture medium is added to each well containing 3.7×104?Bq L-[5-3H]-proline (9.62×1011 Bq/mmol). After 48?h incubation, cells are lysed with 0.5 mL 0.5 mol/L NaOH for 1 h. The resulting solution is neutralized with an equal amount of 0.5 mol/L HCl, and 50 μL are used to measure total proteins with the Bradford method. One volume of 10% TCA is added to the remaining 250 μL and centrifuged at 13,000 g for 15?min at 4°C. The resulting precipitate is dissolved in 100 μL 0.2 mol/L NaOH, and then neutralized with 1 mol/L HCl. The solution is incubated with collagenase buffer (Tris-HCl, pH 7.6 20 mM, and CaCl2 250 mM final concentration) and 10 units of collagenase at 37°C overnight. Then, 150 μL 10% TCA are added and centrifuged at 13000 g for 15?min at 4°C. The resulting supernatant is added to 4 mL of scintillation fluid and the radioactivity is measured in a liquid scintillation counter LS 600 TA.
Synonyms Doxycycline hydrochloride hemiethanolate hemihydrate, WC2031, Doxycycline hyclate
Molecular Weight 512.9
Formula C22H24N2O8·HCl·1/2C2H6O·1/2H2O
CAS No. 24390-14-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 13.89 mg/mL, sonification is recommended.

DMSO: 120mg/mL, sonification is recommended.

TargetMolReferences and Literature

1. Almazin SM, et al. J Periodontol, 2009, 80(6), 999-1005. 2. Breitschwerdt EB, et al. Antimicrob Agents Chemother, 1998, 42(2), 362-368. 3. Perry HD, et al. Cornea, 1993, 12(5), 379-382. 4. Zeidner NS, et al. Antimicrob Agents Chemother, 2004, 48(7), 2697-2699. 5. Meijer LA, et al. Vet Q, 1993, 15(1), 1-5.

Related compound libraries

This product is contained In the following compound libraries:
Microbial Natural Product Library Anti-Bacterial Compound Library Natural Product Library Approved Drug Library Drug Repurposing Compound Library Angiogenesis related Compound Library Antibiotics Library Protease Inhibitor Library Inhibitor Library NO PAINS Compound Library

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Keywords

Doxycycline (hyclate) 24390-14-5 Microbiology/Virology Proteases/Proteasome MMP ribosome Antibacterial Antibiotic U343 anti-proliferative activity SVG MCF 12A WC-2031 antibacterial activity Matrix metalloproteinases 293T LNT-229 hyclate Doxycycline hydrochloride hemiethanolate hemihydrate inhibit WC 2031 Doxycycline Hydrochloride Inhibitor Bacterial WC2031 G55 Doxycycline Doxycycline hyclate inhibitor

 

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