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Acetylcysteine

Catalog No. T0875 CAS 616-91-1

Synonyms: N-Acetyl-L-cysteine, LNAC, NAC

Acetylcysteine (NAC) is an N-acetyl derivative of cysteine, a ROS inhibitor and mucolytic agent. Acetylcysteine induces apoptosis, can be used to reduce mucus thickness, and has anti-influenza viral activity.

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Acetylcysteine, CAS 616-91-1

Pack Size	Availability	Price/USD	Quantity
5 g	In stock	$ 33.00
10 g	In stock	$ 42.00

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Purity: 100%

Purity: 99.94%

Purity: 99.69%

Purity: 99.34%

Purity: 98.98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Acetylcysteine (NAC) is an N-acetyl derivative of cysteine, a ROS inhibitor and mucolytic agent. Acetylcysteine induces apoptosis, can be used to reduce mucus thickness, and has anti-influenza viral activity.
In vitro	METHODS: Human bladder cancer cells T24 were treated with Acetylcysteine (5-50 mM) for 24 h. Cell viability was measured by MTT. RESULTS: Acetylcysteine dose-dependently inhibited the cell viability of T24 cells with an IC50 of 33.33 mM.[1] METHODS: Rat cardiomyocytes H9c2 were treated with Acetylcysteine (2-4 mM) for 12-24 h, and apoptosis was detected by Flow Cytometry. RESULTS: Acetylcysteine dose- and time-dependently induced apoptosis in H9c2 cells. [2]
In vivo	METHODS: To investigate the effects on apoptotic liver injury in mice, Acetylcysteine (150 mg/kg) was administered intraperitoneally to CD-1 mice as a single injection, and GalN/LPS was used to induce hepatic injury 30 min later. RESULTS: Acetylcysteine pretreatment significantly attenuated GalN/LPS-induced hepatocyte apoptosis.Acetylcysteine attenuates GalN/LPS-induced apoptotic liver injury through its potent ROS scavenging and anti-apoptotic effects. [3] METHODS: To assay in vivo activity, Acetylcysteine (500 mg/kg) was administered orally to R6/1 transgenic mice with Huntington's disease (HD) once daily for nine weeks. RESULTS: Chronic Acetylcysteine administration delayed the onset and development of motor deficits in R6/1 mice and also had antidepressant-like effects in both R6/1 and wild-type mice. [4]
Cell Research	For survival experiments, washed cells are resuspended in RPM1 1640 medium and plated in 0.5 mL at a density of 8-10×105 per well in 24 well plastic culture dishes coated with rat tail collagen. To feed, but to avoid loss of floating cells, fresh medium (0.2 mL) is added to the cultures on days 1, 5, and 10. For experiments involving 'primed' PC12 cells, cultures are pretreated for l-2 weeks with NGF in RPM1 1640 medium supplemented with 1% heat-iN-acetylcysteinetivated horse serum. The cells are then washed and passaged into serum-free RPM1 1640 medium.
Animal Research	Rats are randomly allocated into five groups: sham group (n=5), control group with IIR (n=8) and three groups with IIR who are given N-acetylcysteine in different dosages: 150 mg/kg intraperitoneally 5 min before ischemia (n=8, group N-acetylcysteine 150), 300 mg/kg i.p 5 min before ischemia (n=7, group N-acetylcysteine 300), and 150 mg/kg i.p 5 min before ischemia plus 150 mg/kg 5 min before reperfusion (n=7, group N-acetylcysteine 150 + 150). After 4 h of reperfusion, the animals are euthanized by exsanguination from the abdominal aorta.[1]

Synonyms	N-Acetyl-L-cysteine, LNAC, NAC
Molecular Weight	163.19
Formula	C5H9NO3S
CAS No.	616-91-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 31 mg/mL (190 mM), The compound is unstable in solution, please use soon.

Ethanol: 31 mg/mL (190 mM)

H2O: 100 mg/ml (612.78 mM）, Sonification and heating are recommended.

References and Literature

1. Supabphol A, et al. N-acetylcysteine inhibits proliferation, adhesion, migration and invasion of human bladder cancer cells. J Med Assoc Thai. 2009 Sep;92(9):1171-7. 2. Liu Y, et al. N‑acetylcysteine induces apoptosis via the mitochondria‑dependent pathway but not via endoplasmic reticulum stress in H9c2 cells. Mol Med Rep. 2017 Nov;16(5):6626-6633. 3. Wang H, et al. N-acetylcysteine attenuates lipopolysaccharide-induced apoptotic liver damage in D-galactosamine-sensitized mice. Acta Pharmacol Sin. 2007 Nov;28(11):1803-9. 4. Wright DJ, et al. N-Acetylcysteine improves mitochondrial function and ameliorates behavioral deficits in the R6/1 mouse model of Huntington's disease. Transl Psychiatry. 2015 Jan 6;5(1):e492. 5. Farr SA, et al. J Neurochem, 2003, 84(5), 1173-1183. 6. Kalimeris K, et al. N-acetylcysteine ameliorates liver injury in a rat model of intestinal ischemia reperfusion. J Surg Res. 2016 Dec;206(2):263-272. 7. Xu B, Xu J, Cai N, et al. Roflumilast prevents ischemic stroke-induced neuronal damage by restricting GSK3β-mediated oxidative stress and IRE1α/TRAF2/JNK pathway[J]. Free Radical Biology and Medicine. 2020 8. Xu B, Qin Y, Li D, et al. Inhibition of PDE4 protects neurons against oxygen-glucose deprivation-induced endoplasmic reticulum stress through activation of the Nrf-2/HO-1 pathway[J]. Redox Biology. 2020, 28: 101342.

Citations

1. Tang Y, Song H, Wang Z, et al.Repurposing antiparasitic antimonials to noncovalently rescue temperature-sensitive p53 mutations.Cell Reports.2022, 39(2): 110622. 2. Chen T, Leng J, Tan J, et al.Discovery of Novel Potent Covalent Glutathione Peroxidase 4 Inhibitors as Highly Selective Ferroptosis Inducers for the Treatment of Triple-Negative Breast Cancer.Journal of Medicinal Chemistry.2023 3. Liu X, Jin J, Wu Y, et al.Fluoroindole chalcone analogues targeting the colchicine binding site of tubulin for colorectal oncotherapy.European Journal of Medicinal Chemistry.2023: 115540. 4. Li Y, Bao Y, Li Y, et al.RSL3 Inhibits Porcine Epidemic Diarrhea Virus Replication by Activating Ferroptosis.Viruses.2023, 15(10): 2080. 5. Liu X, Sun W, Cao J, et al.Acrolein increases the concentration of intracellular Zn2⁺ by producing mitochondrial reactive oxygen species in A549 cells.Toxicology and Industrial Health.2023: 07482337231198350. 6. Zhu M, Tang X, Gong Z, et al. TAD1822-7 induces ROS-mediated apoptosis of HER2 positive breast cancer by decreasing E-cadherin in an EphB4 dependent manner. Life Sciences. 2021: 119954 7. Xu B, Qin Y, Li D, et al. Inhibition of PDE4 protects neurons against oxygen-glucose deprivation-induced endoplasmic reticulum stress through activation of the Nrf-2/HO-1 pathway. Redox Biology. 2020, 28: 101342 8. Xu B, Xu J, Cai N, et al. Roflumilast prevents ischemic stroke-induced neuronal damage by restricting GSK3β-mediated oxidative stress and IRE1α/TRAF2/JNK pathway. Free Radical Biology and Medicine. 2020 9. Xue J, Liao Q, Luo M, et al. Cigarette smoke-induced oxidative stress activates NRF2 to mediate fibronectin disorganization in vascular formation. Open Biology. 2022, 12(4): 210310 10. Yi Y, Gao K, Lin P, et al. Staphylococcus aureus-Induced Necroptosis Promotes Mitochondrial Damage in Goat Endometrial Epithelial Cells. Animals. 2022, 12(17): 2218.

11. Tang Y, Song H, Wang Z, et al. Repurposing antiparasitic antimonials to noncovalently rescue temperature-sensitive p53 mutations. Cell Reports. 2022, 39(2): 110622 12. Wu X, Ren Y, Wen Y, et al. Deacetylation of ZKSCAN3 by SIRT1 induces autophagy and protects SN4741 cells against MPP+-induced oxidative stress. Free Radical Biology and Medicine. 2022 13. Ma X, Tan X, Yu B, et al. DOCK2 regulates antifungal immunity by regulating RAC GTPase activity. Cellular & Molecular Immunology. 2022: 1-17. 14. Yi Y, Gao K, Zhang L, et al. Zearalenone Induces MLKL-Dependent Necroptosis in Goat Endometrial Stromal Cells via the Calcium Overload/ROS Pathway. International Journal of Molecular Sciences. 2022, 23(17): 10170. 15. Lin L, Chen Y, Li Q, et al. Isoxanthohumol, a component of Sophora flavescens, promotes the activation of the NLRP3 inflammasome and induces idiosyncratic hepatotoxicity. Journal of Ethnopharmacology. 2021: 114796. 16. Zhang Y, Zhang C, Li J, et al. Inhibition of AKT induces p53/SIRT6/PARP1-dependent parthanatos to suppress tumor growth. Cell Communication and Signaling. 2022, 20(1): 1-21 17. Zhang P, Zhang J, Quan H, et al. Effects of butein on human osteosarcoma cell proliferation, apoptosis, and autophagy through oxidative stress. Human & Experimental Toxicology. 2022, 41: 09603271221074346. 18. Yang C, Wang X, To K K W, et al.Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis.Signal Transduction and Targeted Therapy.2024, 9(1): 84.

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Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Human Endogenous Metabolite Library Drug Repurposing Compound Library Inhibitor Library Anti-Cancer Approved Drug Library Anti-Fibrosis Compound Library Pediatric Drug Library Anti-Infection Compound Library NO PAINS Compound Library

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Keywords

Acetylcysteine 616-91-1 Apoptosis Immunology/Inflammation Metabolism Microbiology/Virology NF-Κb ROS Ferroptosis Reactive Oxygen Species Influenza Virus TNF Endogenous Metabolite Inhibitor inhibit N-Acetylcysteine N-Acetyl-L-cysteine LNAC NAC inhibitor

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