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3-Oxo-5β-cholanoic acid

Name: 3-Oxo-5β-cholanoic acid
Brand: TargetMol
SKU: T13502
Price: 41 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T13502Cas No. 1553-56-6

Alias DHLCA, Dehydrolithocholic acid

3-Oxo-5β-cholanoic acid (Dehydrolithocholic acid) is a bile acid metabolite that inhibits TH17 cell differentiation by directly binding to the transcription factor RORγt with a Kd of 1.13 μM.

3-Oxo-5β-cholanoic acid

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Purity: 99.86%

Catalog No. T13502Alias DHLCA, Dehydrolithocholic acidCas No. 1553-56-6

3-Oxo-5β-cholanoic acid (Dehydrolithocholic acid) is a bile acid metabolite that inhibits TH17 cell differentiation by directly binding to the transcription factor RORγt with a Kd of 1.13 μM.

Pack Size	Price	Availability
10 mg	$41	In Stock
25 mg	$63	In Stock
50 mg	$91	In Stock
1 mL x 10 mM (in DMSO)	$46	In Stock

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Purity:99.86%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	3-Oxo-5β-cholanoic acid (Dehydrolithocholic acid) is a bile acid metabolite that inhibits TH17 cell differentiation by directly binding to the transcription factor RORγt with a Kd of 1.13 μM.
In vitro	Treatment with 3-Oxo-5β-cholanoic acid significantly reduces the RORγt reporter's activity, likely by directly interacting with RORγt, thereby inhibiting TH17 cell differentiation by suppressing its transcriptional activity.
In vivo	3-Oxo-5β-cholanoic acid (Dehydrolithocholic acid) (0.3% (w/w); p.o.; for 1 week) significantly reduces the percentage of ileal TH17 cells[1].
Synonyms	DHLCA, Dehydrolithocholic acid

Chemical Properties

Molecular Weight	374.56
Formula	C₂₄H₃₈O₃
Cas No.	1553-56-6
Smiles	C[C@H](CCC(O)=O)[C@H]1CC[C@H]2[C@@H]3CC[C@@H]4CC(=O)CC[C@]4(C)[C@H]3CC[C@]12C
Relative Density.	1.31g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 65 mg/ml (173.54 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.34 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6698 mL	13.3490 mL	26.6980 mL	133.4900 mL
5 mM	0.5340 mL	2.6698 mL	5.3396 mL	26.6980 mL
10 mM	0.2670 mL	1.3349 mL	2.6698 mL	13.3490 mL
20 mM	0.1335 mL	0.6674 mL	1.3349 mL	6.6745 mL
50 mM	0.0534 mL	0.2670 mL	0.5340 mL	2.6698 mL
100 mM	0.0267 mL	0.1335 mL	0.2670 mL	1.3349 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc