CCL5 Protein, Human, Recombinant (His & mucin)

CCL5 Protein, Human, Recombinant (His & mucin) is expressed in HEK293 mammalian cells with His and mucin tag. The predicted molecular weight is 34 kDa and the accession number is D0EI67.

Pack Size	Availability	Price/USD	Quantity
50 μg	5 days	$ 539.00

Pack Size

Availability

Price/USD

Quantity

50 μg

5 days

$ 539.00

Description

CCL5 Protein, Human, Recombinant (His & mucin) is expressed in HEK293 mammalian cells with His and mucin tag. The predicted molecular weight is 34 kDa and the accession number is D0EI67.

Species	Human
Expression System	HEK293 Cells
Tag	C-Mucin-His
Accession Number	D0EI67
Synonyms	D17S136E, SCYA5, SIS-δ, SISd, SIS-delta, RANTES, chemokine (C-C motif) ligand 5, TCP228, eoCP
Construction	The Human RANTES (NP_002976.2) (Met 1-Ser 91) was fused with a C-terminal Human Fractalkine mucin-stalk (Phe 103-Gln 341) followed by a polyhistidine tag.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	34 kDa (predicted)

Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokin ligand 5(CCL5) is chemotactic for T cells, basophils and eosinophils. Chemokin ligand 5(CCL5) has been considered a HIV-supressor secreted by CD8+T cells and other immune cells. Chemokin ligand 5(CCL5) is a key to activating recruit leukocytes into inflammatory sites and in the presence of particular cytokines released by T cells, it can change the NK cells into CHAK cells.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy