FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 with His and AVI tag. The predicted molecular weight is 45.3 kDa. Accession number: NP_004098.1&NP_004039.1
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
20 μg | 5 days | $ 347.00 | |
100 μg | 5 days | $ 1,220.00 |
Description | FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 with His and AVI tag. The predicted molecular weight is 45.3 kDa. Accession number: NP_004098.1&NP_004039.1 |
Species | Human |
Expression System | HEK293 |
Tag | His,AVI |
Accession Number | NP_004098.1&NP_004039.1 |
Synonyms | Fc fragment of IgG, receptor, transporter, alpha, alpha-chain, Fc fragment of IgG, receptor, transporter, α, FCRN, α-chain |
Construction | A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
Molecular Weight | Approxiamtely 45.3 kDa |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
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Please read the User Guide of Recombinant Proteins for more specific information.
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & Avi), Biotinylated Fc fragment of IgG, receptor, transporter, alpha alpha-chain Fc fragment of IgG, receptor, transporter, α FCRN α-chain a-chain recombinant recombinant-proteins proteins protein