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WS6

Name: WS6
Brand: TargetMol
SKU: T2106
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2106Cas No. 1421227-53-3

WS6, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.

WS6

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Purity: 99.95%

Catalog No. T2106Cas No. 1421227-53-3

WS6, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$30	In Stock	In Stock
2 mg	$34	In Stock	In Stock
5 mg	$46	In Stock	In Stock
10 mg	$74	In Stock	In Stock
25 mg	$139	In Stock	In Stock
50 mg	$225	In Stock	In Stock
100 mg	$333	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$57	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.95%

Color:White to Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	WS6, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.
Targets&IC50	Proliferation (β cells):0.28 μM
In vitro	In the RIP-DTA mouse model with β-cell ablation, oral administration of WS6 (50 mg/kg) promotes β-cell proliferation, thereby ameliorating diabetes.
In vivo	In R7T1 cells (EC50=0.28 μM), WS6 induces cell proliferation through the action on the Erb3 binding protein-1 and IκB kinase signaling pathways.
Kinase Assay	PARP assays are conducted in a buffer containing 50 mM Tris (pH 8.0), 1 mM DTT, 1.5 μM [3H]NAD+?(1.6 μCi/mmol), 200 nM biotinylated histone H1, 200 nM slDNA, and 1 nM PARP-1 or 4 nM PARP-2 enzyme. Reactions are terminated with 1.5 mM benzamide, transferred to streptavidin Flash plates, and counted using a TopCount microplate scintillation counter.
Cell Research	R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference)

Chemical Properties

Molecular Weight	568.59
Formula	C₂₉H₃₁F₃N₆O₃
Cas No.	1421227-53-3
Smiles	C(C1=C(C(F)(F)F)C=C(NC(CC2=CC=C(OC=3C=C(NC(=O)C4CC4)N=CN3)C=C2)=O)C=C1)N5CCN(C)CC5
Relative Density.	1.374 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (163.56 mM), Sonication is recommended.

Ethanol: 93 mg/mL (163.56 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (5.8 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.7587 mL	8.7937 mL	17.5874 mL	87.9368 mL
5 mM	0.3517 mL	1.7587 mL	3.5175 mL	17.5874 mL
10 mM	0.1759 mL	0.8794 mL	1.7587 mL	8.7937 mL
20 mM	0.0879 mL	0.4397 mL	0.8794 mL	4.3968 mL
50 mM	0.0352 mL	0.1759 mL	0.3517 mL	1.7587 mL
100 mM	0.0176 mL	0.0879 mL	0.1759 mL	0.8794 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc