Thiamine nitrate (Vitamin B1 nitrate) is an essential vitamin, and can enhance normal neuronal actives.

In a study examining the effect of dietary Thiamine on homozygous knockout (KO) and knock-in (KI) mice compared to wild-type (WT) mice, it was found that at 7 weeks, Thiamine levels in the blood of KO and KI mice fed a conventional diet were significantly reduced to 0.058±0.051 μM and 0.126±0.092 μM, respectively, in contrast to WT mice (0.796±0.259 μM). Further, when subjected to a Thiamine-restricted diet (0.60 mg/100 g food), both KO and KI mice showed a drastic decrease in blood Thiamine concentrations, dropping to 0.010±0.009 and 0.010±0.006 μM after 5 and 14 days, correspondingly, compared to WT counterparts (0.609±0.288 μM). Additionally, Thiamine levels in the brain homogenates of KO and KI mice were also observed to be lower than in WT mice when fed a conventional diet, with concentrations being 1.33±0.96 nmol/g and 2.16±1.55 nmol/g wet weight, respectively, versus 3.81±2.18 nmol/g in WT. Notably, the Thiamine concentration in brain homogenates of KO and KI mice decreased further to 0.95±0.72 and 1.11±0.24 nmol/g wet weight respectively after 5 and 14 days on a Thiamine-restricted diet, compared to WT mice (3.65±1.02 nmol/g wet weight), ahead of showcasing a disease phenotype.