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P 22077

Name: P 22077
Brand: TargetMol
SKU: T2424
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2424Cas No. 1247819-59-5

Alias P22077

P 22077 (P22077) is an inhibitor of ubiquitin-specific protease USP7 with EC50 of 8.6 μM. It also inhibits the closely related USP47.

P 22077

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Purity: 99.64%

Catalog No. T2424Alias P22077Cas No. 1247819-59-5

P 22077 (P22077) is an inhibitor of ubiquitin-specific protease USP7 with EC50 of 8.6 μM. It also inhibits the closely related USP47.

Pack Size	Price	USA Warehouse	Global Warehouse
2 mg	$32	In Stock	In Stock
5 mg	$52	In Stock	In Stock
10 mg	$83	In Stock	In Stock
25 mg	$167	In Stock	In Stock
50 mg	$319	In Stock	In Stock
100 mg	$476	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$91	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.64%

Color:White to Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	P 22077 (P22077) is an inhibitor of ubiquitin-specific protease USP7 with EC50 of 8.6 μM. It also inhibits the closely related USP47.
Targets&IC50	USP7:8.6 μM(EC50)
In vivo	P22077, administered at concentrations of 15-45 mM to cell lysates, inhibits a subset of DUBs (deubiquitinating enzymes) and prompts tumor cell death with an EC50 in the low micromolar range. Treatment with P22077 during the release from a G1/S arrest induced by hydroxyurea in U2OS cells results in a dose-dependent decrease in claspin protein levels, accompanied by reduced phosphorylation of serine 317 on Chk1. Furthermore, after P22077 exposure, quantitative mass spectrometry reveals diminished levels of E3 ubiquitin ligase components RBX1, DCAF7, DCAF11, and DNA damage-binding protein 1. While displaying negligible activity against DEN1 and SENP2 core, P22077 robustly inhibits USP7 with an IC50 of 8 μM, indicating selective inhibition towards USP7 and closely related DUB, USP47, over a range of DUBs, cysteine proteases, and other protease families in vitro.
Synonyms	P22077

Chemical Properties

Molecular Weight	315.32
Formula	C₁₂H₇F₂NO₃S₂
Cas No.	1247819-59-5
Smiles	CC(=O)c1cc(c(Sc2ccc(F)cc2F)s1)[N+]([O-])=O
Relative Density.	1.53 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 31.5 mg/mL (99.9 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 2 mg/mL (6.34 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.1714 mL	15.8569 mL	31.7138 mL	158.5691 mL
5 mM	0.6343 mL	3.1714 mL	6.3428 mL	31.7138 mL
10 mM	0.3171 mL	1.5857 mL	3.1714 mL	15.8569 mL
20 mM	0.1586 mL	0.7928 mL	1.5857 mL	7.9285 mL
50 mM	0.0634 mL	0.3171 mL	0.6343 mL	3.1714 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc