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Nimbolide

Name: Nimbolide
Brand: TargetMol
SKU: T16324
Price: 65 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16324 Copy Product Info

Purity: 99.4%

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Nimbolide, a triterpenoid derived from neem leaves and flowers, is a natural product and a CDK4/CDK6/NF-κB inhibitor that inhibits multiple signaling pathways including Wnt and PI3K-Akt, possesses antitumor activity, and can induce tumor cell apoptosis.

Cas No. 25990-37-8

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Pack Size	Price	USA Stock	Global Stock
1 mg	$65	In Stock	In Stock
5 mg	$186	In Stock	In Stock
10 mg	$322	In Stock	In Stock
25 mg	$662	In Stock	In Stock
50 mg	$912	-	In Stock
100 mg	$1,230	-	In Stock
200 mg	$1,600	-	In Stock
1 mL x 10 mM (in DMSO)	$201	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:99.4%

Appearance:Solid

Color:White to Yellow

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COA HNMR HPLC

Product Introduction

Nimbolide AI Summary

Nimbolide exhibits a range of bioactivities, suggesting its potential as a drug candidate. It shows significant antiproliferative activity against various human cancer cell lines (OVCAR5, PC3, A549, HOP62, SW60, HT29, MDA-MB-231, MCF7) with differing IC50 values, indicating its ability to inhibit cancer cell proliferation. Additionally, it demonstrates cytotoxicity against human and mouse cells with ED50 values between 0.25 to 0.53 µg/mL, within 48 to 72 hours of exposure. The compound also shows antimalarial activity against Plasmodium falciparum, with an IC50 of 1.74 µg/mL and an EC50 of 0.00095 µg/mL against the K1 strain. Moreover, Nimbolide has antifeedant properties against Spodoptera litura larvae. In pharmacokinetic studies, it reaches a Cmax of 32.8 nM in Sprague-Dawley rats when administered orally at 10 mg/kg, and 11907.06 nM intravenously at the same dose. The compound inhibits NDM-1 with an IC50 of 1340.0 nM and has shown moderate toxicity in animal models, with corresponding LD50 values. These multifaceted activities highlight its promise for further preclinical evaluation..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Nimbolide, a triterpenoid derived from neem leaves and flowers, is a natural product and a CDK4/CDK6/NF-κB inhibitor that inhibits multiple signaling pathways including Wnt and PI3K-Akt, possesses antitumor activity, and can induce tumor cell apoptosis.
Targets & IC50	Parasites:8.13-9.4 μM, MCF-7 cells proliferation:5.04 ± 0.25 μM, N1E-115 cells viability:4-10 μM, MDA-MB-231 cells proliferation:1.97 ± 0.24 μM
In vitro	Methods:BV-2 mouse microglial cells were pretreated with Nimbolide (125, 250, 500 nM) for 30 minutes, then co-incubated with LPS (100 ng/mL) for 24 hours. Detection was performed using ELISA, Griess assay, and Western blot. Results: Nimbolide concentration-dependently reduced levels of TNFα, IL-6, IFNγ, NO/iNOS, and PGE2/COX-2, inhibited NF-κB and MAPK pathways, and activated the Nrf2 antioxidant mechanism.[1] Methods: Nine H. pylori strains including G27, 26695, and J99 were incubated with Nimbolide at 0.625–40 μg/mL for 24 hours. In vitro activity was evaluated through MIC/MBC determination and bactericidal kinetics assays. Results: Nimbolide exhibited MIC values of 1.25–2.5 μg/mL and MBC values of 2.5–10 μg/mL against tested strains, demonstrating time- and concentration-dependent bactericidal effects. It showed synergistic activity at low pH and was effective against multidrug-resistant strains.[2] Methods: Human bladder cancer EJ and 5637 cells were incubated with Nimbolide at 0, 0.5, 1, and 3 μmol/L for 12 hours. Detection was performed using MTT assay, cell counting, flow cytometry, wound healing assay, Transwell invasion assay, gelatin zymography, and EMSA. Results: Nimbolide concentration-dependently inhibited cell viability (IC₅₀ approximately 3 μmol/L), induced G2/M phase arrest, promoted JNK phosphorylation, inhibited p38MAPK and AKT phosphorylation, and reduced MMP-9 activity as well as NF-κB, AP-1, and Sp-1 transcription factor binding activity.[3]
In vivo	Methods: A Freund's complete adjuvant-induced arthritis model was established in male Wistar rats. Nimbolide was administered orally at a dose of 20 mg/kg/day with DMSO as the vehicle control, continuously until day 25. Results: Nimbolide significantly reduced joint scores, paw swelling, and organ indices, alleviated histopathological damage, and downregulated expression of inflammatory factors including TNF-α and IL-6 as well as iNOS and NF-κB proteins.[4] Methods: DSS-induced acute colitis and IL-10⁻/⁻ chronic colitis mouse models were employed. Nimbolide was administered by gavage at doses of 0.2 and 1 mg/kg/day with DMSO as the vehicle, continuously for 7 or 14 days. Results: Nimbolide significantly improved weight loss, colon shortening, disease activity index, and histological scores, and inhibited IκBα phosphorylation in colon tissue.[5]

Chemical Properties

Molecular Weight	466.52
Formula	C₂₇H₃₀O₇
Cas No.	25990-37-8
Smiles	C[C@]12[C@]3([C@]([C@@]4([C@](C)([C@@H]1CC(OC)=O)C=5[C@](O4)(C[C@H](C5C)C=6C=COC6)[H])[H])(OC(=O)[C@]3(C)C=CC2=O)[H])[H]
Relative Density.	1.32 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Keep away from direct sunlight,Keep away from moisture,Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (128.61 mM), Sonication and heating are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.14 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1435 mL	10.7177 mL	21.4353 mL	107.1765 mL
5 mM	0.4287 mL	2.1435 mL	4.2871 mL	21.4353 mL
10 mM	0.2144 mL	1.0718 mL	2.1435 mL	10.7177 mL
20 mM	0.1072 mL	0.5359 mL	1.0718 mL	5.3588 mL
50 mM	0.0429 mL	0.2144 mL	0.4287 mL	2.1435 mL
100 mM	0.0214 mL	0.1072 mL	0.2144 mL	1.0718 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.