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Nimbolide

Name: Nimbolide
Brand: TargetMol
SKU: T16324
Price: 65 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16324Cas No. 25990-37-8

Nimbolide suppresses CDK4/CDK6 kinase activity. Nimbolide inhibits the NF-κB, Wnt, PI3K-Akt, MAPK, and JAK-STAT signaling pathways. Nimbolide causes apoptosis through the inactivation of NF-κB. Nimbolide is a triterpene derived from the leaves and flowers of neem (Azadirachta indica L).

Nimbolide

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Purity: 99.4%

Catalog No. T16324Cas No. 25990-37-8

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$65	In Stock	In Stock
5 mg	$186	In Stock	In Stock
10 mg	$322	In Stock	In Stock
25 mg	$662	In Stock	In Stock
50 mg	$912	-	In Stock
100 mg	$1,230	-	In Stock
200 mg	$1,600	-	In Stock
1 mL x 10 mM (in DMSO)	$201	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

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Purity:99.4%

Appearance:Solid

Color:White to Yellow

Resource Download

COA HNMR HPLC

Product Introduction

Nimbolide AI Summary

Nimbolide exhibits a range of bioactivities, suggesting its potential as a drug candidate. It shows significant antiproliferative activity against various human cancer cell lines (OVCAR5, PC3, A549, HOP62, SW60, HT29, MDA-MB-231, MCF7) with differing IC50 values, indicating its ability to inhibit cancer cell proliferation. Additionally, it demonstrates cytotoxicity against human and mouse cells with ED50 values between 0.25 to 0.53 µg/mL, within 48 to 72 hours of exposure. The compound also shows antimalarial activity against Plasmodium falciparum, with an IC50 of 1.74 µg/mL and an EC50 of 0.00095 µg/mL against the K1 strain. Moreover, Nimbolide has antifeedant properties against Spodoptera litura larvae. In pharmacokinetic studies, it reaches a Cmax of 32.8 nM in Sprague-Dawley rats when administered orally at 10 mg/kg, and 11907.06 nM intravenously at the same dose. The compound inhibits NDM-1 with an IC50 of 1340.0 nM and has shown moderate toxicity in animal models, with corresponding LD50 values. These multifaceted activities highlight its promise for further preclinical evaluation..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Nimbolide suppresses CDK4/CDK6 kinase activity. Nimbolide inhibits the NF-κB, Wnt, PI3K-Akt, MAPK, and JAK-STAT signaling pathways. Nimbolide causes apoptosis through the inactivation of NF-κB. Nimbolide is a triterpene derived from the leaves and flowers of neem (Azadirachta indica L).
Targets&IC50	Parasites:8.13-9.4 μM, N1E-115 cells viability:4-10 μM, MCF-7 cells proliferation:5.04 ± 0.25 μM, MDA-MB-231 cells proliferation:1.97 ± 0.24 μM

Chemical Properties

Molecular Weight	466.52
Formula	C₂₇H₃₀O₇
Cas No.	25990-37-8
Smiles	C[C@]12[C@]3([C@]([C@@]4([C@](C)([C@@H]1CC(OC)=O)C=5[C@](O4)(C[C@H](C5C)C=6C=COC6)[H])[H])(OC(=O)[C@]3(C)C=CC2=O)[H])[H]
Relative Density.	1.32 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture,store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (128.61 mM), Sonication and heating are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (2.14 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.1435 mL	10.7177 mL	21.4353 mL	107.1765 mL
5 mM	0.4287 mL	2.1435 mL	4.2871 mL	21.4353 mL
10 mM	0.2144 mL	1.0718 mL	2.1435 mL	10.7177 mL
20 mM	0.1072 mL	0.5359 mL	1.0718 mL	5.3588 mL
50 mM	0.0429 mL	0.2144 mL	0.4287 mL	2.1435 mL
100 mM	0.0214 mL	0.1072 mL	0.2144 mL	1.0718 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc