Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-CDK4 Antibody (6D570)
😃Good
Catalog No. TMAB-00406
Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-CDK4 Antibody (6D570)
Anti-CDK4 Antibody (6D570)
😃Good
Catalog No. TMAB-00406Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Questions
View MoreResource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | PSK-J3, cyclin-dependent kinase 4, CMM3 |
| Clone | 6D570 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Sample: Lane 1: Embryo (Mouse) Lysate at 40 μg Lane 2: Placenta (Mouse) Lysate at 40 μg Lane 3: Heart (Mouse) Lysate at 40 μg Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 5: Heart (Rat) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Jurkat (Human) Cell Lysate at 30 μg Lane 8: Hela (Human) Cell Lysate at 30 μg Lane 9: MCF-7 (Human) Cell Lysate at 30 μg Lane 10: A431 (Human) Cell Lysate at 30 μg Primary: Anti-Cdk4 (TMAB-00406) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 32 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide: human Cdk4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cdk4,Cdks,Cdks,Cdks |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-CDK4 Antibody (6D570) | purchase Anti-CDK4 Antibody (6D570) | Anti-CDK4 Antibody (6D570) cost | order Anti-CDK4 Antibody (6D570) | Anti-CDK4 Antibody (6D570) molecular weight
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.