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Anti-CDK4 Antibody (6D570)
😃Good
Catalog No. TMAB-00406
Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-CDK4 Antibody (6D570)
Anti-CDK4 Antibody (6D570)
😃Good
Catalog No. TMAB-00406Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Alias | PSK-J3, cyclin-dependent kinase 4, CMM3 |
| Clone | 6D570 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Sample: Lane 1: Embryo (Mouse) Lysate at 40 μg Lane 2: Placenta (Mouse) Lysate at 40 μg Lane 3: Heart (Mouse) Lysate at 40 μg Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 5: Heart (Rat) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Jurkat (Human) Cell Lysate at 30 μg Lane 8: Hela (Human) Cell Lysate at 30 μg Lane 9: MCF-7 (Human) Cell Lysate at 30 μg Lane 10: A431 (Human) Cell Lysate at 30 μg Primary: Anti-Cdk4 (TMAB-00406) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 32 kDa |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide: human Cdk4 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cdk4,Cdks,Cdks,Cdks |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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