Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

6D570

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Sample:
Lane 1: Embryo (Mouse) Lysate at 40 μg
Lane 2: Placenta (Mouse) Lysate at 40 μg
Lane 3: Heart (Mouse) Lysate at 40 μg
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 5: Heart (Rat) Lysate at 40 μg
Lane 6: Cerebrum (Rat) Lysate at 40 μg
Lane 7: Jurkat (Human) Cell Lysate at 30 μg
Lane 8: Hela (Human) Cell Lysate at 30 μg
Lane 9: MCF-7 (Human) Cell Lysate at 30 μg
Lane 10: A431 (Human) Cell Lysate at 30 μg
Primary:
Anti-Cdk4 (TMAB-00406) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 32 kDa

ICC/IF=1:50-200; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:500-2000

Monoclonal

KLH conjugated synthetic peptide: human Cdk4

Human

CDK4

Cyclin-dependent kinase 4 Cyclin-dependent kinase 4

Cdk4,Cdks,Cdks,Cdks

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.