Anti-CDK4 Polyclonal Antibody is a Rabbit antibody targeting CDK4. Anti-CDK4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Pig,Cow)

1. Sample:
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-CDK4 (TMAB-00405) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
2. Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CDK4 Polyclonal Antibody, Unconjugated (TMAB-00405) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Brain (Rat) Lysate at 30 μg
Colon carcinoma (Human) lysate at 30 μg
Primary: Anti-CDK4 (TMAB-00405) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/3000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
4. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-CDK4 (TMAB-00405) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
5. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDK4) Polyclonal Antibody, Unconjugated (TMAB-00405) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CDK4) polyclonal Antibody, Unconjugated (TMAB-00405) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDK4) Polyclonal Antibody, Unconjugated (TMAB-00405) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (Red line): Rabbit Anti-CDK4 antibody (TMAB-00405);
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (green line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1 μg/test.
9. Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CDK4) Polyclonal Antibody, Unconjugated (TMAB-00405) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CDK4

Human

Cdk4,Cdks,Cdks,Cdks