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Neohesperidin Dihydrochalcone

Name: Neohesperidin Dihydrochalcone
Brand: TargetMol
SKU: T2767
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2767Cas No. 20702-77-6

Alias NHDC, Neohesperidin DC, NCI-c60764

Neohesperidin Dihydrochalcone (Neohesperidin DC) is an artificial sweetener derived from citrus.

Neohesperidin Dihydrochalcone

🥰Excellent

Purity: 99.97%

Catalog No. T2767Alias NHDC, Neohesperidin DC, NCI-c60764Cas No. 20702-77-6

Neohesperidin Dihydrochalcone (Neohesperidin DC) is an artificial sweetener derived from citrus.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.97%

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Neohesperidin Dihydrochalcone (Neohesperidin DC) is an artificial sweetener derived from citrus.
In vivo	Administration of neohesperidin dihydrochalcone significantly reduces the activities of AST and ALT, both key markers of liver damage. It also inhibits the expression of NF-κB, IL-6, IL-1β, and TNF-α proteins, which are elevated in the liver of PQ-treated mice, indicating its potential protective effects on liver inflammation and damage[3]. Furthermore, studies on Wistar Crl:(WI)WU BR rats to assess the embryotoxicity and teratogenicity of neohesperidin dihydrochalcone reveal no adverse effects at dietary levels up to 5%, the highest dose investigated. At this level, rats consumed approximately 3.3 g/kg body weight/day[4], demonstrating the compound's safety at significant dosage levels.
Cell Research	WST-8 dye is used in the cell viability assay. HIT-T15 and HUVEC cells are grown and maintained in Dulbecco's modified Eagle's medium, supplemented with 10% fetal bovine calf serum. 1000 cells in each well are incubated with various concentrations of neohesperidin dihydrochalcone (50, 100, 500 μM, 1 mM) and other compounds. After treating HIT-T15 and HUVEC cells with 500 μM HOCl, WST-8 dye is added to each well, and the absorbance is detected at 420 nm with microplate reader[1].
Synonyms	NHDC, Neohesperidin DC, NCI-c60764

Chemical Properties

Molecular Weight	612.58
Formula	C₂₈H₃₆O₁₅
Cas No.	20702-77-6
Smiles	O([C@H]1[C@H](O[C@H]2[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O2)[C@@H](O)[C@H](O)[C@@H](CO)O1)C3=CC(O)=C(C(CCC4=CC(O)=C(OC)C=C4)=O)C(O)=C3
Relative Density.	1.61 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 100 mg/mL (163.24 mM), Sonication is recommended.

DMSO: 50 mg/mL (81.62 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.26 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.6324 mL	8.1622 mL	16.3244 mL	81.6220 mL
5 mM	0.3265 mL	1.6324 mL	3.2649 mL	16.3244 mL
10 mM	0.1632 mL	0.8162 mL	1.6324 mL	8.1622 mL
20 mM	0.0816 mL	0.4081 mL	0.8162 mL	4.0811 mL
50 mM	0.0326 mL	0.1632 mL	0.3265 mL	1.6324 mL

Ethanol

	1mg	5mg	10mg	50mg
100 mM	0.0163 mL	0.0816 mL	0.1632 mL	0.8162 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc