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Methyl Cholate

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Catalog No. T2744Cas No. 1448-36-8

Alias Cholic acid methyl ester

Methyl Cholate is the methyl ester form of bile acid, which is a major bile acid produced by the liver and synthesized from cholesterol.

Methyl Cholate

🥰Excellent

Purity: 96.22%

Catalog No. T2744Alias Cholic acid methyl esterCas No. 1448-36-8

Methyl Cholate is the methyl ester form of bile acid, which is a major bile acid produced by the liver and synthesized from cholesterol.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$42	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$46	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:96.22%

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COA HNMR

Product Introduction

Bioactivity

Description	Methyl Cholate is the methyl ester form of bile acid, which is a major bile acid produced by the liver and synthesized from cholesterol.
Kinase Assay	Collagenase assay uses the synthetic thiol ester substrate Ac-Pro-Leu-Gly-SCH(i-Bu)CO-Leu-Gly-OEt at pH 6.5. The collagenase concentration is 1-2 nM, and the substrate concentrations are from 0.1 to 0.7 nM. Km?is found to vary between 1.5 and 4 mM.
Synonyms	Cholic acid methyl ester

Chemical Properties

Molecular Weight	422.6
Formula	C₂₅H₄₂O₅
Cas No.	1448-36-8
Smiles	O[C@H]1[C@]2([C@]3([C@@](C)([C@@]([C@@H](CCC(OC)=O)C)(CC3)[H])[C@@H](O)C[C@@]2([C@]4(C)[C@](C1)(C[C@H](O)CC4)[H])[H])[H])[H]
Relative Density.	1.141 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (130.15 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3663 mL	11.8315 mL	23.6630 mL	118.3152 mL
5 mM	0.4733 mL	2.3663 mL	4.7326 mL	23.6630 mL
10 mM	0.2366 mL	1.1832 mL	2.3663 mL	11.8315 mL
20 mM	0.1183 mL	0.5916 mL	1.1832 mL	5.9158 mL
50 mM	0.0473 mL	0.2366 mL	0.4733 mL	2.3663 mL
100 mM	0.0237 mL	0.1183 mL	0.2366 mL	1.1832 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc