Shopping Cart

Remove All
Your shopping cart is currently empty

Col003

Name: Col003
Brand: TargetMol
SKU: T10860
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T10860Cas No. 328565-16-8

Col003 is a selective and potent inhibitor of Hsp47, competitively binding to the collagen-binding site on Hsp47 (IC50: 1.8 μM). It is useful for researching fibrosis.

Col003

🥰Excellent

Purity: 99.03%

Catalog No. T10860Cas No. 328565-16-8

Col003 is a selective and potent inhibitor of Hsp47, competitively binding to the collagen-binding site on Hsp47 (IC50: 1.8 μM). It is useful for researching fibrosis.

Pack Size	Price	Availability
2 mg	$39	In Stock
5 mg	$64	In Stock
10 mg	$109	In Stock
25 mg	$228	In Stock
50 mg	$369	In Stock
100 mg	$571	In Stock
200 mg	$819	In Stock
500 mg	$1,230	In Stock
1 mL x 10 mM (in DMSO)	$64	In Stock

Add to Cart

Add to Quotation

Bulk & Custom

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.03%

Resource Download

COA HPLC HNMR

Product Introduction

Bioactivity

Description	Col003 is a selective and potent inhibitor of Hsp47, competitively binding to the collagen-binding site on Hsp47 (IC50: 1.8 μM). It is useful for researching fibrosis.
Targets&IC50	HSP47:1.8 μM
In vitro	A molecule AK778 and its cleavage product Col003 competitively inhibited the interaction and caused the inhibition of collagen secretion by destabilizing the collagen triple helix. Structural information obtained with NMR analysis revealed that Col003 competitively binds to the collagen-binding site on Hsp47. Col003 has an inhibitory effect on the interaction of Hsp47 with collagen in Hsp47 KO / MEFs. Col003 (0.01-100 μM) exhibits dose-dependent effects on the interactions of Hsp47 with collagen (IC50: 1.8 μM for Hsp47). Col003 (100 μM; 20-60 mins) has an inhibitory effect on collagen secretion and accumulation. It inhibits collagen secretion by wild-type MEFs and the secretion is not abolished completely. Col003 (100 μM) degrades α,α′-dipyridyl collagen secretion completely by incubation with trypsin at a high temperature (50 °C for 15 min), but is resistant to trypsin digestion at 37 °C.

Chemical Properties

Molecular Weight	257.24
Formula	C₁₄H₁₁NO₄
Cas No.	328565-16-8
Smiles	Oc1c(C=O)cc(Cc2ccccc2)cc1[N+]([O-])=O
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 188 mg/mL (730.84 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.77 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8874 mL	19.4371 mL	38.8742 mL	194.3710 mL
5 mM	0.7775 mL	3.8874 mL	7.7748 mL	38.8742 mL
10 mM	0.3887 mL	1.9437 mL	3.8874 mL	19.4371 mL
20 mM	0.1944 mL	0.9719 mL	1.9437 mL	9.7186 mL
50 mM	0.0777 mL	0.3887 mL	0.7775 mL	3.8874 mL
100 mM	0.0389 mL	0.1944 mL	0.3887 mL	1.9437 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc