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AT-533

Name: AT-533
Brand: TargetMol
SKU: T67836
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T67836Cas No. 908112-37-8

AT-533 is a potent inhibitor of Hsp90 and HSV. AT-533 blocks the HIF-1α/VEGF/VEGFR-2 signaling pathway, leading to suppress tumor growth and angiogenesis. AT-533 also inhibits the activation of the downstream pathways, including Erk1/2, FAK, Akt/mTOR/p70S6K,. AT-533 inhibits the cell migration, invasion, and the tube formation of human umbilical vein endothelial cells (HUVECs).

AT-533

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Purity: 99.67%

Catalog No. T67836Cas No. 908112-37-8

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$33	In Stock	In Stock
5 mg	$79	In Stock	In Stock
10 mg	$125	In Stock	In Stock
25 mg	$230	In Stock	In Stock
50 mg	$343	In Stock	In Stock
100 mg	$496	In Stock	In Stock
200 mg	$667	-	In Stock
1 mL x 10 mM (in DMSO)	$83	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.67%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	AT-533 is a potent inhibitor of Hsp90 and HSV. AT-533 blocks the HIF-1α/VEGF/VEGFR-2 signaling pathway, leading to suppress tumor growth and angiogenesis. AT-533 also inhibits the activation of the downstream pathways, including Erk1/2, FAK, Akt/mTOR/p70S6K,. AT-533 inhibits the cell migration, invasion, and the tube formation of human umbilical vein endothelial cells (HUVECs).
In vitro	AT-533 (0-1350 nM; 24h or 48h) inhibits 20 ng/mL VEGF-induced tube formation, cell migration, and HUVEC invasion. Additionally, AT-533 (2 μM or 75 μM; 24h) hinders the HIF-1α/VEGF signaling pathway in hypoxia-induced breast cancer cells and inhibits Akt/mTOR/p70S6K, Erk1/2, and FAK phosphorylation. It shows anti-angiogenic activity on the chorionic villus (CAM) model at 10 nM and 50 nM over 48h. Furthermore, AT-533 (0.5 μM; 2 or 4 hours) reduces TNF-α, IL-1β, and IL-6 production induced by HSV-1 in RAW264.7 and BV2 cells [1, 2].
In vivo	AT-533 (10 mg/kg; intraperitoneal injection; once daily for 21 days) inhibited the expression of HIF-1α/VEGF signaling pathway-related proteins in a mouse MDA-MB-231 xenograft model of breast cancer [1]. AT-533 (1, 2, 4 mg/kg; intraperitoneal injection; once daily for 30 days) did not cause death, appetite loss, weight loss, or adverse reactions in subacute toxicity tests of Sprague Dawley rats [3].

Chemical Properties

Molecular Weight	410.51
Formula	C₂₃H₃₀N₄O₃
Cas No.	908112-37-8
Smiles	Cc1nn(c2CC(C)(C)CC(=O)c12)-c1ccc(C(N)=O)c(N[C@H]2CC[C@H](O)CC2)c1
Relative Density.	1.36 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (146.16 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.87 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4360 mL	12.1800 mL	24.3599 mL	121.7997 mL
5 mM	0.4872 mL	2.4360 mL	4.8720 mL	24.3599 mL
10 mM	0.2436 mL	1.2180 mL	2.4360 mL	12.1800 mL
20 mM	0.1218 mL	0.6090 mL	1.2180 mL	6.0900 mL
50 mM	0.0487 mL	0.2436 mL	0.4872 mL	2.4360 mL
100 mM	0.0244 mL	0.1218 mL	0.2436 mL	1.2180 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc