Squalene (AddaVax) is an intermediate in cholesterol synthesis. Squalene has a variety of pharmacological properties, such as antilipemic, hepatoprotective, anti-atherosclerotic, cardioprotective, antioxidant, antitumor and antifungal.

JURKAT cells:26.22 μg/ml, HepG2 cells:100 μg/ml

METHODS: Human MCF7, NCI-H460, and SF-268 cells were treated with Squalene (0-50 μM) for 72 hours, and MTS reduction assay was used to detect cell growth inhibition.
RESULTS: Squalene did not inhibit the proliferation of MCF7, NCI-H460 and SF-268 cells (IC50>50 μM). [1]
METHODS: MCF10A epithelial cells were treated with Squalene (12.5, 50, and 200 μM) for 24 hours, and ROS levels were measured using a microplate reader.
RESULTS: Squalene reduced intracellular ROS levels. [2]

METHODS: To study the effect of Squalene on lipid parameters, mice were fed a diet containing Squalene (0.25-1 g/kg) for 11 weeks.
RESULTS: Squalene promoted the changes of HDL-cholesterol and paraoxonase 1, and decreased the reactive oxygen species in lipoproteins and plasma malondialdehyde levels. [3]