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SL327

Catalog No. T2708   CAS 305350-87-2
Synonyms: SL 327, SL-327

SL327 is a selective inhibitor for MEK1/2 with IC50 of 0.18 μM/0.22 μM; able to transport through the blood-brain barrier.

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SL327 Chemical Structure
SL327, CAS 305350-87-2
Pack Size Availability Price/USD Quantity
5 mg In stock $ 48.00
10 mg In stock $ 68.00
25 mg In stock $ 113.00
50 mg In stock $ 173.00
100 mg In stock $ 278.00
500 mg In stock $ 969.00
1 mL * 10 mM (in DMSO) In stock $ 54.00
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Purity: 100%
Purity: 99.74%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description SL327 is a selective inhibitor for MEK1/2 with IC50 of 0.18 μM/0.22 μM; able to transport through the blood-brain barrier.
Targets&IC50 MEK2:0.22 μM, MEK1:0.18 μM
Kinase Assay The ligand binding competition assays are performed. Cytosolic cell extracts from Hepa-1 cells are generated by the resuspension of the cell pellets in HEDG buffer [25 mM Hepes, 1 mM EDTA, 1 mM dithiothreitol, and 10% (v/v) glycerol, pH 7.5] containing 0.4 mM leupeptin, 4 mg/mL aprotinin, and 0.3 mM phenylmethylsulfonyl fluoride, homogenization, and centrifugation at 100,000 g for 45 min. Aliquots of the supernatant (120 μg) are incubated at room temperature for 2 h with the indicated concentrations of Pifithrin-α in the presence of 3 nM [3H]TCDD in HEDG buffer. After incubation on ice with hydroxyapatite for 30 min, HEDG buffer with 0.5% Tween 80 is added. The samples are centrifuged, washed twice, resuspended in 0.2 mL of scintillation fluid, and subjected to scintillation counting. Nonspecific binding is determined using a 150-fold molar excess of TCDF and subtracted from the total binding to obtain the specific binding. The specific binding is reported relative to [3H]TCDD alone[2].
Synonyms SL 327, SL-327
Molecular Weight 335.35
Formula C16H12F3N3S
CAS No. 305350-87-2

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 33.5 mg/mL (100 mM)

Ethanol: 16.8 mg/mL (50 mM)

TargetMolReferences and Literature

1. Scherle PA, wt al. J Biol Chem. 2000, 275(47), 37086-37092. 2. Atkins CM, et al. Nat Neurosci, 1998, 1(7), 602-609. 3. Selcher JC, et al. Learn Mem, 1999, 6(5), 478-490. 4. Valjent E, J Neurosci, 2000, 20(23), 8701-8709.

TargetMolCitations

1. Zhu H, Liu X, Wang X, et al.Gβγ subunit inhibitor decreases DOM-induced head twitch response via the PLCβ/IP3/Ca2+/ERK and cAMP signaling pathways.European Journal of Pharmacology.2023: 176038.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Tyrosine Kinase Inhibitor Library Toxic Compound Library Anti-Aging Compound Library Autophagy Compound Library Glutamine Metabolism Compound Library MAPK Inhibitor Library Anti-Pancreatic Cancer Compound Library Anti-Prostate Cancer Compound Library Reprogramming Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SL327 305350-87-2 Cell Cycle/Checkpoint DNA Damage/DNA Repair MAPK MEK DNA/RNA Synthesis Mitogen-activated protein kinase kinase inhibit Inhibitor MAPKK SL 327 MAP2K SL-327 inhibitor

 

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