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Anti-JUN Polyclonal Antibody

😃Good
Catalog No. TMAB-01011
Alias V-jun avian sarcoma virus 17 oncogene homolog, Transcription factor Jun, Transcription factor AP-1 subunit Jun, Proto-oncogene c-Jun, p39, JUN, Activator protein 1 (AP1)

Anti-JUN Polyclonal Antibody is a Rabbit antibody targeting JUN. Anti-JUN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-JUN Polyclonal Antibody

Anti-JUN Polyclonal Antibody

😃Good
Catalog No. TMAB-01011Alias V-jun avian sarcoma virus 17 oncogene homolog, Transcription factor Jun, Transcription factor AP-1 subunit Jun, Proto-oncogene c-Jun, p39, JUN, Activator protein 1 (AP1)
Anti-JUN Polyclonal Antibody is a Rabbit antibody targeting JUN. Anti-JUN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-JUN Polyclonal Antibody is a Rabbit antibody targeting JUN. Anti-JUN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasV-jun avian sarcoma virus 17 oncogene homolog, Transcription factor Jun, Transcription factor AP-1 subunit Jun, Proto-oncogene c-Jun, p39, JUN, Activator protein 1 (AP1)
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Sheep)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-C-Jun Polyclonal Antibody, Unconjugated (TMAB-01011) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Liver (Rat) lysate at 30 μg;
Brain (Rat) lysates at 30 μg;
Primary: Anti-C-jun/AP-1 (TMAB-01011) at 1:200;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 36 kDa
Observed band size: 36 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C-jun) Polyclonal Antibody, Unconjugated (TMAB-01011) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C-jun) Polyclonal Antibody, Unconjugated (TMAB-01011) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Blank control (blue line): HepG2 (blue).
Primary Antibody (green line): Rabbit Anti-C-jun antibody
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C-jun) Polyclonal Antibody, Unconjugated (TMAB-01011) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
7. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Lung (Mouse) Lysate at 40 μg
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 4: Hela (Human) Cell Lysate at 30 μg
Primary: Anti-C-jun (TMAB-01011) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/36 kDa
Observed band size: 45 kDa
8. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-C-jun antibody (TMAB-01011)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundC-jun (Oncoprotein C-jun) is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Transcription factor AP-1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Transcription factors,SMADs,Leucine Zipper,Transcription Factors,TLR Signaling,Phosphoprotein ELISA kits,SMADs
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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