Powder: -20°C for 3 years
In solvent: -80°C for 2 years
SGI-1776 has been used in trials studying the treatment of Prostate Cancer, Non-Hodgkins Lymphoma, and Relapsed/Refractory Leukemias.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | $ 49.00 | |
5 mg | In stock | $ 99.00 | |
10 mg | In stock | $ 175.00 | |
25 mg | In stock | $ 320.00 | |
50 mg | In stock | $ 461.00 | |
100 mg | In stock | $ 830.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 100.00 |
Description | SGI-1776 has been used in trials studying the treatment of Prostate Cancer, Non-Hodgkins Lymphoma, and Relapsed/Refractory Leukemias. |
Targets&IC50 | FLT3:44 nM, Pim1:7 nM, Pim3:69 nM, Pim2:363 nM |
Kinase Assay | Kinase Assays: Kinase inhibition is measured by the use of radiometric assays performed by KinaseProfiler service. Assays contain a peptide substrate, known purified recombinant human kinases, gamma-labeled ATP, magnesium ion, and a fixed concentration (1 μM) of SGI-1776. In a final reaction volume of 25 μL, 5 to 10 mU of Pim1/2/3 is incubated with 8 mM of MOPS, pH 7.0; 0.2 mM ethylene diamine tetraacetic acid; 100 μM KKRNRTLTV;10 mM MgAcetate; and [γ-32P-ATP] . The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μL of a 3% phosphoric acid solution. Then, 10 μL of the reaction is spotted onto a P30 filtermat and washed 3 times for 5 minutes in 75 mM phosphoric acid and once in methanol before it is dried and measured via a scintillation counter. |
Cell Research | Cells are cultured in IMDM (ATCC) supplemented with 10% FBS and grown in a 37oC incubator with 5% CO2. Cells are routinely tested for Mycoplasma infection using a commercially available kit. Cells are treated with DMSO or various concentrations of SGI-1776 for 24 hours. Cells (1×106) are washed, then resuspended in 100 μL of annexin binding buffer, mixed with 5 μL of FITC solution and 5 μL of propidium iodide (PI; 50 μg/mL) solution. For each sample, 1×104 cells are measured using a Becton Dickinson FACSCalibur flow cytometer. (Only for Reference) |
Synonyms | SGI-1776 free base, Pim-Kinase Inhibitor IX |
Molecular Weight | 405.425 |
Formula | C20H22F3N5O |
CAS No. | 1025065-69-3 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 75 mg/mL (185 mM)
Ethanol: 75 mg/mL (185 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
SGI-1776 1025065-69-3 Angiogenesis Apoptosis JAK/STAT signaling Chromatin/Epigenetic Tyrosine Kinase/Adaptors Autophagy FLT Pim Inhibitor Pim kinases SGI-1776 free base SGI1776 inhibit Pim-Kinase Inhibitor IX SGI 1776 inhibitor