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SGI-1776

Catalog No. T3078 CAS 1025065-69-3

Synonyms: SGI-1776 free base, Pim-Kinase Inhibitor IX

SGI-1776 has been used in trials studying the treatment of Prostate Cancer, Non-Hodgkins Lymphoma, and Relapsed/Refractory Leukemias.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

SGI-1776, CAS 1025065-69-3

Pack Size	Availability	Price/USD
2 mg	In stock	$ 49.00
5 mg	In stock	$ 99.00
10 mg	In stock	$ 175.00
25 mg	In stock	$ 320.00
50 mg	In stock	$ 461.00
100 mg	In stock	$ 830.00
1 mL * 10 mM (in DMSO)	In stock	$ 100.00

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Purity: 99.03%

Purity: 100%

Purity: 99.84%

Purity: 99.3%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	SGI-1776 has been used in trials studying the treatment of Prostate Cancer, Non-Hodgkins Lymphoma, and Relapsed/Refractory Leukemias.
Targets&IC50	FLT3:44 nM, Pim1:7 nM, Pim3:69 nM, Pim2:363 nM
Kinase Assay	Kinase Assays: Kinase inhibition is measured by the use of radiometric assays performed by KinaseProfiler service. Assays contain a peptide substrate, known purified recombinant human kinases, gamma-labeled ATP, magnesium ion, and a fixed concentration (1 μM) of SGI-1776. In a final reaction volume of 25 μL, 5 to 10 mU of Pim1/2/3 is incubated with 8 mM of MOPS, pH 7.0; 0.2 mM ethylene diamine tetraacetic acid; 100 μM KKRNRTLTV;10 mM MgAcetate; and [γ-32P-ATP] . The reaction is initiated by the addition of the MgATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of 5 μL of a 3% phosphoric acid solution. Then, 10 μL of the reaction is spotted onto a P30 filtermat and washed 3 times for 5 minutes in 75 mM phosphoric acid and once in methanol before it is dried and measured via a scintillation counter.
Cell Research	Cells are cultured in IMDM (ATCC) supplemented with 10% FBS and grown in a 37oC incubator with 5% CO2. Cells are routinely tested for Mycoplasma infection using a commercially available kit. Cells are treated with DMSO or various concentrations of SGI-1776 for 24 hours. Cells (1×106) are washed, then resuspended in 100 μL of annexin binding buffer, mixed with 5 μL of FITC solution and 5 μL of propidium iodide (PI; 50 μg/mL) solution. For each sample, 1×104 cells are measured using a Becton Dickinson FACSCalibur flow cytometer. (Only for Reference)

Synonyms	SGI-1776 free base, Pim-Kinase Inhibitor IX
Molecular Weight	405.425
Formula	C20H22F3N5O
CAS No.	1025065-69-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 75 mg/mL (185 mM)

Ethanol: 75 mg/mL (185 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Chen LS, et al. Blood, 2011, 118(3), 693-702. 2. Chen LS, et al. Blood, 2009, 114(19), 4150-7. 3. Batra V, et al. Pediatr Blood Cancer, 2012, 59(4), 749-752.

Related compound libraries

This product is contained In the following compound libraries：

Epigenetics Compound Library Kinase Inhibitor Library Clinical Compound Library Anti-Cancer Compound Library Tyrosine Kinase Inhibitor Library Anti-Prostate Cancer Compound Library Anti-Pancreatic Cancer Compound Library Immunology/Inflammation Compound Library Anti-Cancer Active Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

SGI-1776 1025065-69-3 Angiogenesis Apoptosis JAK/STAT signaling Chromatin/Epigenetic Tyrosine Kinase/Adaptors Autophagy FLT Pim Inhibitor Pim kinases SGI-1776 free base SGI1776 inhibit Pim-Kinase Inhibitor IX SGI 1776 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.