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Anti-VEGFR1/FLT-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01955
Alias VEGFR1, VEGF R1, FRT, Flt-1, FLT1, FLT
Anti-VEGFR1/FLT-1 Polyclonal Antibody is a Rabbit antibody targeting VEGFR1/FLT-1. Anti-VEGFR1/FLT-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-VEGFR1/FLT-1 Polyclonal Antibody
Anti-VEGFR1/FLT-1 Polyclonal Antibody
😃Good
Catalog No. TMAB-01955Alias VEGFR1, VEGF R1, FRT, Flt-1, FLT1, FLT
Anti-VEGFR1/FLT-1 Polyclonal Antibody is a Rabbit antibody targeting VEGFR1/FLT-1. Anti-VEGFR1/FLT-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-VEGFR1/FLT-1 Polyclonal Antibody is a Rabbit antibody targeting VEGFR1/FLT-1. Anti-VEGFR1/FLT-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | VEGFR1, VEGF R1, FRT, Flt-1, FLT1, FLT |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (TMAB-01955) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293) Primary: Anti-VEGFR1 (TMAB-01955) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 147 kDa Observed band size: 140 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Endosome. Note=Autophosphorylation promotes ubiquitination and endocytosis；Isoform 2: Secreted；Isoform 3: Secreted；Isoform 4: Secreted；Isoform 5: Cytoplasm (Potential)；Isoform 6: Cytoplasm (Potential)；Isoform 7: Cytoplasm (Potential). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes. |
| Immunogen | KLH conjugated synthetic peptide: mouse VEGFR1 |
| Antigen Species | Mouse |
| Biology Area | Response to hypoxia,VEGF,Angiogenic growth factors,Growth factors,Endothelial Cell Markers,VEGF Receptors,Endothelium,Hypoxia,Cancer,VEGF,Tyrosine Kinases |
| Molecular Weight | Theoretical: 147 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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