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Puerarin

Catalog No. T2815 CAS 3681-99-0

Synonyms: Kakonein

Puerarin (Kakonein), also known as Kakonein, is a member of the class of compounds known as isoflavonoid C-glycosides. It is a 5-HT2C receptor antagonist.

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Puerarin, CAS 3681-99-0

Pack Size	Availability	Price/USD
5 mg	In stock	$ 47.00
10 mg	In stock	$ 80.00
25 mg	In stock	$ 163.00
50 mg	In stock	$ 247.00
100 mg	In stock	$ 372.00
500 mg	In stock	$ 869.00
1 mL * 10 mM (in DMSO)	In stock	$ 89.00

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Purity: 99.84%

Purity: 98.9%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Puerarin (Kakonein), also known as Kakonein, is a member of the class of compounds known as isoflavonoid C-glycosides. It is a 5-HT2C receptor antagonist.
In vitro	Administering 300 mg/kg of Puerarin daily through oral ingestion to rats on a high-cholesterol diet significantly reduces the increase in total cholesterol levels in both serum and liver caused by the high-cholesterol diet.
In vivo	Puerarin, at a dose of 25 μM, induces dose-dependent growth inhibition in HT-29 cells. This effect is accompanied by an increase in bax and a decrease in c-myc and bcl-2.
Kinase Assay	STAT3-dependent dual-luciferase assay: HCT-116 cells are transiently transfected with reporter plasmid having the STAT3-binding element for regulating luciferase assay. Cells are treated with Cryptotanshinone for 24 hours at a concentration range of 0.2 to 50 μM. After treatment, cells are harvested in 20 μL of passive lysis buffer and luciferase activity is evaluated by the Dual Luciferase Reporter Assay kit on Wallac Victor2. The concentration of Cryptotanshinone that inhibits the luciferase activity by 50% represents IC50 value.
Cell Research	RAW264.7 cells are maintained at subconfluence in 95% air and 5% CO2 humidified atmosphere maintained at 37°C. The medium used for routine subculture is Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, penicillin (100 units/mL) and streptomycin (100 μg/ mL). An MTT assay is used to measure the viability of the cells after treatment with puerarin. After the supernatants are removed for nitrite determination, cells are incubated at 37°C with MTT (0.05 mg/mL) for 4 h, and the optical density is measured at 540 nm. The concentrations of puerarin are10, 20, 40 and 100 μM[1].
Source	Natural Products > Fabaceae > Pueraria

Synonyms	Kakonein
Molecular Weight	416.38
Formula	C21H20O9
CAS No.	3681-99-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 80 mg/mL (192.1 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Overstreet DH, et al. Pharmacol Biochem Behav, 2003, 75(3), 619-625. 2. Yan LP, et al. Life Sci, 2006, 79(4), 324-330. 3. Yu Z, et al. Cancer Lett, 2006, 238(1), 53-60. 4. Liang Y, Xu Z, Wu X, et al. Inhibition of hyperpolarization-activated cyclic nucleotide-gated channels with natural flavonoid quercetin[J]. Biochemical and Biophysical Research Communications. 2020

Citations

1. Wang X, He K, Ma L, et al. Puerarin attenuates isoproterenol‑induced myocardial hypertrophy via inhibition of the Wnt/β‑catenin signaling pathway. Molecular Medicine Reports. 2022, 26(4): 1-13. 2. Liang Y, Xu Z, Wu X, et al. Inhibition of hyperpolarization-activated cyclic nucleotide-gated channels with natural flavonoid quercetin. Biochemical and Biophysical Research Communications. 2020

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Approved Drug Library Inhibitor Library Drug Repurposing Compound Library Anti-Cancer Clinical Compound Library Membrane Protein-targeted Compound Library Anti-Cancer Drug Library GPCR Compound Library Anti-Neurodegenerative Disease Compound Library Anti-Cancer Compound Library Polyphenolic Natural Product Library

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Keywords

Puerarin 3681-99-0 GPCR/G Protein Neuroscience 5-HT Receptor 5-hydroxytryptamine Receptor inhibit Kakonein Serotonin Receptor Inhibitor inhibitor

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