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Clozapine N-Oxide

Name: Clozapine N-Oxide
Brand: TargetMol
SKU: T4494
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4494Cas No. 34233-69-7

Clozapine N-oxide is the major metabolite of Clozapine and is blood-brain barrier permeable. Clozapine N-oxide is an agonist of DREADDs and activates the DREADD receptors hM3Dq and hM4Di. Clozapine N-oxide is also a dopamine antagonist and selective muscarinic M4 receptor agonist.

Clozapine N-Oxide

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Purity: 99.94%

Catalog No. T4494Cas No. 34233-69-7

Pack Size	Price	Availability
2 mg	$30	In Stock
5 mg	$45	In Stock
10 mg	$76	In Stock
25 mg	$126	In Stock
50 mg	$187	In Stock
100 mg	$278	In Stock

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Purity:99.94%

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COA HNMR HPLC

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Product Introduction

Bioactivity

Description	Clozapine N-oxide is the major metabolite of Clozapine and is blood-brain barrier permeable. Clozapine N-oxide is an agonist of DREADDs and activates the DREADD receptors hM3Dq and hM4Di. Clozapine N-oxide is also a dopamine antagonist and selective muscarinic M4 receptor agonist.
In vitro	METHODS: hM4Di-transduced primary embryonic rat neuronal cultures were treated with Clozapine N-oxide (0.01-1000 nM), and activity on hM4Di receptors was detected by FLIPR assay. RESULTS: The EC50 value for Clozapine N-oxide was 8.1 nM. [1] METHODS: WT and hM3Dq-transfected mouse embryoma cells ATDC5 were treated with Clozapine N-oxide (750 nM) to detect intracellular calcium signaling. RESULTS: Administration of Clozapine N-oxide resulted in immediate coordinated induction of single [Ca2+]i spines in hM3Dq-ATDC5 cells, and only spontaneous [Ca2+]i signaling was observed in Clozapine N-oxide-treated WT controls. [2]
In vivo	METHODS: To investigate potential effects on sleep, Clozapine N-oxide (1-10 mg/kg) was injected intraperitoneally into C57BL/6J mice. RESULTS: Clozapine N-oxide altered sleep in mice. Sleep was analyzed using electroencephalography (EEG) and electromyography (EMG), revealing dose-dependent suppression of rapid eye movement (REM) sleep, changes in EEG spectral power during non-rapid eye movement (NREM) sleep, and alterations in sleep structure. [3] METHODS: To investigate the effects on reward-seeking behavior, Clozapine N-oxide (0.1-10 mg/kg) was administered intraperitoneally to C57BL6/JJcl mice. RESULTS: The off-target effects of commonly used doses of Clozapine N-oxide on motivated reward seeking were negligible. [4]

Chemical Properties

Molecular Weight	342.82
Formula	C₁₈H₁₉ClN₄O
Cas No.	34233-69-7
Smiles	CN1(=O)CCN(C=2C=3C(NC=4C(N2)=CC(Cl)=CC4)=CC=CC3)CC1
Relative Density.	1.36g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | Shipping with blue ice.

Solubility Information

H2O: 1 mg/mL (2.92 mM), Sonication is recommended.

Ethanol: 10 mg/mL (29.17 mM), Sonication is recommended.

DMSO: 60 mg/mL (175.02 mM), The compound is unstable in solution, please use soon.

In Vivo Formulation

Saline: 25 mg/mL (72.92 mM), In vivo: Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.9170 mL	14.5849 mL	29.1698 mL	145.8491 mL

Ethanol/DMSO

	1mg	5mg	10mg	50mg
5 mM	0.5834 mL	2.9170 mL	5.8340 mL	29.1698 mL
10 mM	0.2917 mL	1.4585 mL	2.9170 mL	14.5849 mL
20 mM	0.1458 mL	0.7292 mL	1.4585 mL	7.2925 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0583 mL	0.2917 mL	0.5834 mL	2.9170 mL
100 mM	0.0292 mL	0.1458 mL	0.2917 mL	1.4585 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

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