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Palifosfamide

Catalog No. T4350 CAS 31645-39-3

Synonyms: ZIO-201, Isophosphamide mustard, Isophosphoramide mustard

Palifosfamide (Isophosphamide mustard) lysine (ZIO-201) is a stable form of palifosfamide. Palifosfamide lysine has broad activity in sarcoma lines in vitro. The IC50 ranges from 2.25 to 6.75 μM for most cell lines except OS222 (IC50=31.5 μM).

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Palifosfamide, CAS 31645-39-3

Pack Size	Availability	Price/USD
1 mg	In stock	$ 70.00
2 mg	In stock	$ 98.00
5 mg	In stock	$ 172.00
10 mg	In stock	$ 289.00
25 mg	In stock	$ 452.00
50 mg	In stock	$ 596.00
100 mg	In stock	$ 863.00
1 mL * 10 mM (in DMSO)	In stock	$ 189.00

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Purity: 97%

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Description	Palifosfamide (Isophosphamide mustard) lysine (ZIO-201) is a stable form of palifosfamide. Palifosfamide lysine has broad activity in sarcoma lines in vitro. The IC50 ranges from 2.25 to 6.75 μM for most cell lines except OS222 (IC50=31.5 μM).
Targets&IC50	OS222:31.5 μM
In vitro	Differential gene expression of ALDH3A1 but not ALDH1A1 is noted in the OS31 xenograft. Stabilized palifosfamide administered to mice suppresses MX-1 tumor growth by greater than 80% with 17% complete antitumor responses. Oral bioavailability in rats is 48-73% of parenteral administration, and antitumor activity in mice is equivalent by both routes. Treatment with palifosfamide-tris combined with docetaxelor doxorubicin at optimal regimens results in complete tumor regression in 62-75% of mice.
Cell Research	Palifosfamide is dissolved in phosphate buffered saline (PBS). Cells are plated in 96-well microtiter plates with approximately 500 cells per well in 100 μL of media. After 24 h of incubation at 37°C, cells are treated with increasing concentrations of palifosfamide lysine in separate plates either as a single-day treatment or three consecutive days of treatment, with fresh drug being added each day. The plates are incubated for 72 h at 37°C with 5% CO2. After 72 h, 250 μg of MTT is added to each well and incubated at 37°C for 6 h. MTT is converted to formazine crystals by mitochondria of viable cells, which are then dissolved in 100 μL of dimethyl sulfoxide. Optical density is measured at 595 nm.
Animal Research	Mouse: CB17 female SCID mice are used in the study. Once the tumors reached 50–150 mm3, mice are randomized into control and treatment groups (5-8 mice/group) for each tumor line. Cyclophosphamide is administered at the dose of 150 mg/kg intraperitoneally once a week for 6 weeks. Palifosfamide lysine is administered intravenously at the maximum tolerated dose of 100 mg/kg for three consecutive days as a one-time treatment and serial tumor volumes are determined over the next 6 weeks. Mice are sacrificed at the end of the experiment.

Synonyms	ZIO-201, Isophosphamide mustard, Isophosphoramide mustard
Molecular Weight	221.02
Formula	C4H11Cl2N2O2P
CAS No.	31645-39-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 42 mg/mL

References and Literature

1. Hingorani P, et al. Preclinical activity of palifosfamide lysine (ZIO-201) in pediatric sarcomas including oxazaphosphorine-resistant osteosarcoma. Cancer Chemother Pharmacol. 2009 Sep;64(4):733-40. 2. Jones B, et al. Anticancer activity of stabilized palifosfamide in vivo: schedule effects, oral bioavailability, and enhanced activity with docetaxel and doxorubicin. Anticancer Drugs. 2012 Feb;23(2):173-84.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Tyrosine Kinase Inhibitor Library Anti-Cancer Drug Library Drug Repurposing Compound Library Bioactive Compounds Library Max Bioactive Compound Library Anti-Lung Cancer Compound Library ReFRAME Related Library Target-Focused Phenotypic Screening Library

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Keywords

Palifosfamide 31645-39-3 DNA Damage/DNA Repair Metabolism DNA Alkylation DNA Alkylator/Crosslinker Drug Metabolite ZIO-201 Isophosphamide mustard inhibit ZIO 201 Inhibitor Isophosphoramide mustard IPM ZIO201 inhibitor

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