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Nodinitib-1

Nodinitib-1
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Purity:99.98%
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Nodinitib-1

Catalog No. T6591Cas No. 799264-47-4
Nodinitib-1 (CID-1088438) is a potent and selective NOD1 inhibitor with an IC50 of 0.56 μM.
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Pack SizePrice/USDAvailabilityQuantity
5 mg$50In Stock
10 mg$61In Stock
25 mg$143In Stock
50 mg$225In Stock
100 mg$373In Stock
200 mg$563In Stock
1 mL x 10 mM (in DMSO)$57In Stock
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Product Introduction

Bioactivity
Description
Nodinitib-1 (CID-1088438) is a potent and selective NOD1 inhibitor with an IC50 of 0.56 μM.
In vitro
ML130 has shown selective inhibition of NOD1-induced NF-κB activation in HEK293 cells with no cytotoxicity and is selected as a probe candidate molecule. ML130 is also confirmed in secondary assays by selectively inhibiting NOD1-dependent IL-8 secretion and also selectively inhibiting the NOD1-dependent pathway to NF-κB activation. [1] In another research, ML130 is proved to cause conformational changes of NOD1 in vitro and alter NOD1 subcellular targeting in cells, providing chemical probes for interrogating mechanisms regulating NOD1 activity and tools for exploring the roles of NOD1 in various infectious and inflammatory diseases. [2]
Kinase Assay
NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.
Cell Research
Hepatic toxicity of compounds is determined with Fa2N-4 immortalized human hepatocytes using the ATP-lite 1-step assay. Fa2N-4 cells are seeded at 50,000 cells/well, and incubated with a range of concentrations of the test compound (0.01 μM-50 μM) in MFE support medium for 24 h at 37 ℃, 5% CO2. At the end of the experiment D-luciferin and luciferase are added. The emitted luminescent signal produced as a result of the reaction with cellular ATP is captured on the Infinite M200 plate reader. The concentration of each compound that killed 50% of the cells (LC50) is calculated by non-linear regression analysis using a log (inhibitior) vs response equation with a variable slope, using the statistic software package Prism4.(Only for Reference)
AliasML130, CID-1088438
Chemical Properties
Molecular Weight287.34
FormulaC14H13N3O2S
Cas No.799264-47-4
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
Ethanol: 2.9 mg/mL (10 mM)
DMSO: 28.7 mg/mL (100 mM)
Solution Preparation Table
DMSO/Ethanol
1mg5mg10mg50mg
1 mM3.4802 mL17.4010 mL34.8020 mL174.0099 mL
5 mM0.6960 mL3.4802 mL6.9604 mL34.8020 mL
DMSO
1mg5mg10mg50mg
10 mM0.3480 mL1.7401 mL3.4802 mL17.4010 mL
20 mM0.1740 mL0.8700 mL1.7401 mL8.7005 mL
50 mM0.0696 mL0.3480 mL0.6960 mL3.4802 mL
100 mM0.0348 mL0.1740 mL0.3480 mL1.7401 mL

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