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Catalog No. T6591   CAS 799264-47-4
Synonyms: ML130, CID-1088438

Nodinitib-1 is a potent and selective NOD1 inhibitor with an IC50 of 0.56 μM.

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Nodinitib-1, CAS 799264-47-4
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Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Nodinitib-1 is a potent and selective NOD1 inhibitor with an IC50 of 0.56 μM.
Targets&IC50 NOD1:0.56 μM
In vitro ML130 has shown selective inhibition of NOD1-induced NF-κB activation in HEK293 cells with no cytotoxicity and is selected as a probe candidate molecule. ML130 is also confirmed in secondary assays by selectively inhibiting NOD1-dependent IL-8 secretion and also selectively inhibiting the NOD1-dependent pathway to NF-κB activation. [1] In another research, ML130 is proved to cause conformational changes of NOD1 in vitro and alter NOD1 subcellular targeting in cells, providing chemical probes for interrogating mechanisms regulating NOD1 activity and tools for exploring the roles of NOD1 in various infectious and inflammatory diseases. [2]
Kinase Assay NOD1 Dose Response assay: Day 1 Procedure 1) Harvest HEK-293-T NFKB-Luc at 100% confluency at 100% confluency. 2) Add NOD assay media with Multidrop. 3) Spin down plates at 1000 rpm for 1 min in centrifuge. 4) Serial compound dilutions. 5) Add gamma-tri-DAP to cell suspension at 0.75 ug/mL. 6) Seed 13000 cells/well in 4 uL/well to full plate HEK-293-T NFKB-Luc to TC-treated plate. 7) Spin down plates 500 RPM for 5 min on centrifuge. 8) Lid Plates. Sandwich 4 plates between 2 lidded 384 plates filled with Water. 9) Wrap plates securely in single layer of Plastic Wrap. 10) Incubate overnight (14 hours) in 37 ℃ and 5% CO2 incubator. Day 2 Procedure 1) Add 3 ul/well of SteadyGlo solution with Multidrop. 2) Shake plates on a plate shaker for 20 min. 3) Spin plates 1000 RPM for 1 min using centrifuge. 4) Read luminescence.IC50 values are calculated using GraphPad Prism 5.0.The average Z' for the screen is 0.6, the signal to background is 11.1, signal to noise is 78.6 and signal to window is 6.0.
Cell Research Hepatic toxicity of compounds is determined with Fa2N-4 immortalized human hepatocytes using the ATP-lite 1-step assay. Fa2N-4 cells are seeded at 50,000 cells/well, and incubated with a range of concentrations of the test compound (0.01 μM-50 μM) in MFE support medium for 24 h at 37 ℃, 5% CO2. At the end of the experiment D-luciferin and luciferase are added. The emitted luminescent signal produced as a result of the reaction with cellular ATP is captured on the Infinite M200 plate reader. The concentration of each compound that killed 50% of the cells (LC50) is calculated by non-linear regression analysis using a log (inhibitior) vs response equation with a variable slope, using the statistic software package Prism4.(Only for Reference)
Synonyms ML130, CID-1088438
Molecular Weight 287.34
Formula C14H13N3O2S
CAS No. 799264-47-4


Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 2.9 mg/mL (10 mM)

DMSO: 28.7 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Khan PM et al. ACS Med Chem Lett, 2011, 2(10), 780-785. 2. Correa RG et al. Chem Biol, 2011, 18(7), 825-832.


1. Zhang Y, Li N, Yuan G, et al. Upregulation of NOD1 and NOD2 contribute to cancer progression through the positive regulation of tumorigenicity and metastasis in human squamous cervical cancer. BMC medicine. 2022, 20(1): 1-15.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Compound Library HIF-1 Signaling Pathway Compound Library Cytokine Inhibitor Library Anti-Obesity Compound Library Inhibitor Library Immuno-Oncology Compound Library Immunology/Inflammation Compound Library NF-κB Signaling Compound Library Apoptosis Compound Library Anti-Aging Compound Library

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Nodinitib-1 799264-47-4 NF-Kb信号通路 凋亡 免疫与炎症 NOD NOD-like Receptor (NLR) TNF Nodinitib1 CID1088438 Inhibitor CID-1088438 Nodinitib 1 ML-130 ML 130 CID 1088438 ML130 inhibit inhibitor