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Malachite green oxalate

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Catalog No. TD0099Cas No. 2437-29-8

Malachite green oxalate is a triphenylmethane dye which can be used to detect the release of phosphate in enzymatic reactions.

Malachite green oxalate

Malachite green oxalate

Copy Product Info
🥰Excellent
Catalog No. TD0099Cas No. 2437-29-8
Malachite green oxalate is a triphenylmethane dye which can be used to detect the release of phosphate in enzymatic reactions.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
100 mg$41In StockIn Stock
1 mL x 10 mM (in DMSO)$45In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Purity:N/A
Appearance:solid
Color:Green
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Product Introduction

Malachite green oxalate AI Summary
Malachite green oxalate exhibits bioactivity in multiple assays concerning cell viability, ATP levels, cytotoxicity, and interactions with specific cellular receptors and enzymes. It shows potency in modulating several biological pathways and receptors including the thyroid hormone receptor, steroid receptor coregulator, hormone dehydrogenases, DNA helicases, and signaling pathways such as ERK and NF-kB. Additionally, it has demonstrated cytotoxic effects on various cell lines and the potential to influence cellular metabolic activities. Notably, this compound also shows efficacy in blocking the entry of viruses like Ebola and Lassa in specific assays..
Note: Summary generated by AI. Data source: ChEMBL
Bioactivity
Description
Malachite green oxalate is a triphenylmethane dye which can be used to detect the release of phosphate in enzymatic reactions.
In vitro
Bacterial staining experiment
Material preparation: Malachite green oxalate stain, bacterial smear to be tested, alcohol lamp, distilled water, safranin stain, etc.
Experimental steps:
a. Make a bacterial smear, dry it naturally and fix it with the flame of an alcohol lamp.
b. Add Malachite green oxalate stain, cover the smear, heat it until steam comes out, keep it for about 5 minutes, add appropriate stain to prevent drying, and then rinse with distilled water.
c. Re-stain with safranin stain for 2-3 minutes, rinse with distilled water and dry.
d. Observe under a microscope, the bacteria are dyed green, and structures such as spores can be clearly observed.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.
Chemical Properties
Molecular Weight463.51
FormulaC23H25N2·C2HO4·1/2C2H2O4
Cas No.2437-29-8
SmilesO=C(C(O)=O)[O-].O=C(C(O)=O)O.CN(C)C(C=C1)=CC=C1/C(C2=CC=CC=C2)=C3C=C/C(C=C/3)=[N+](C)/C.O=C(C(O)=O)[O-].CN(C)C(C=C4)=CC=C4/C(C5=CC=CC=C5)=C6C=C/C(C=C/6)=[N+](C)/C
Relative Density.no data available
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
DMSO: 8.67 mg/mL (18.71 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween-80+45% Saline: 0.5 mg/mL (1.08 mM), Sonication is recommended.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.1575 mL10.7873 mL21.5745 mL107.8725 mL
5 mM0.4315 mL2.1575 mL4.3149 mL21.5745 mL
10 mM0.2157 mL1.0787 mL2.1575 mL10.7873 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
mg/kg
g
μL
2 Enter the in vivo formulation:
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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