Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Irinotecan (CPT-11) is an antineoplastic enzyme inhibitor primarily used in the treatment of colorectal cancer. It is a derivative of camptothecin that inhibits the action of topoisomerase I. Irinotecan prevents religation of the DNA strand by binding to topoisomerase I-DNA complex, and causes double-strand DNA breakage and cell death.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
25 mg | In stock | $ 42.00 | |
50 mg | In stock | $ 59.00 | |
100 mg | In stock | $ 91.00 | |
500 mg | In stock | $ 229.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | Irinotecan (CPT-11) is an antineoplastic enzyme inhibitor primarily used in the treatment of colorectal cancer. It is a derivative of camptothecin that inhibits the action of topoisomerase I. Irinotecan prevents religation of the DNA strand by binding to topoisomerase I-DNA complex, and causes double-strand DNA breakage and cell death. |
Cell Research | Irinotecan is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. To determine the effects of Irinotecan in combination with 5-FU, the MTT assay is used. Depending on the cell lines, 10,000 to 20,000 cells per well are seeded in 96-well plates and incubated for 24 h in complete medium. On day 2, cells are incubated in the absence or presence of Irinotecan for 30 min followed by 5-FU for 24 h. After another 24 h in complete medium without any additives, MTT reagent is added on day 4 to initiate the assay and the cells are incubated for an additional 4 h at 37°C. After removal of the medium and dissolving the crystals with acidified isopropanol, the samples are analyzed using an ELISA plate reader at 570 nm. The value at 650 nm is subtracted as background[1]. |
Source |
Synonyms | CPT-11, Topotecin, (+)-Irinotecan |
Molecular Weight | 586.68 |
Formula | C33H38N4O6 |
CAS No. | 97682-44-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 32 mg/mL (54.5 mM), sonification is recommended.
You can also refer to dose conversion for different animals. More
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Irinotecan 97682-44-5 Autophagy DNA Damage/DNA Repair Topoisomerase Inhibitor CPT-11 Topotecin CPT 11 CPT11 inhibit (+)-Irinotecan inhibitor