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Clitocine

Catalog No. T30969 CAS 105798-74-1

Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.

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Clitocine, CAS 105798-74-1

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.
In vitro	For premature stop codon readthrough activity, Clitocine incorporation into mRNA is required, and the presence of clitocine at the third position of a premature stop codon is sufficient to promote robust readthrough[2].Clitocine enhances TRAIL lethality in LS411N and SW620 cells, with concentrations ranging from 0 to 0.8 μM over a 24-hour period. Additionally, at a concentration of 0.2 μM for 36 hours, Clitocine significantly potentiates TRAIL-mediated apoptosis[1].
In vivo	Induced by Citocine (0.3-3 mg/kg; s.c.; five times per week), p53 inhibits CAOV-33p53-UAA136 tumor growth in a xenograft model[2].

Molecular Weight	287.23
Formula	C9H13N5O6
CAS No.	105798-74-1

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 80 mg/mL (278.52 mM), Sonication is recommended.

References and Literature

1. Sun JG, et al. Clitocine potentiates TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation. Apoptosis. 2016;21(10):1144-1157. 2. Friesen WJ, et al. The nucleoside analog clitocine is a potent and efficacious readthrough agent. RNA. 2017;23(4):567-577. 3. Sun JG, Xiang J, Zeng XL, Li X, Wu P, Fung KP, Liu FY. Clitocine induces apoptosis and enhances the lethality of ABT-737 in human colon cancer cells by disrupting the interaction of Mcl-1 and Bak. Cancer Lett. 2014 Dec 28;355(2):253-63. doi: 10.1016/j.canlet.2014.09.024. Epub 2014 Oct 7. PubMed PMID: 25304383. 4. Sun JG, Li H, Li X, Zeng X, Wu P, Fung KP, Liu FY. Clitocine targets Mcl-1 to induce drug-resistant human cancer cell apoptosis in vitro and tumor growth inhibition in vivo. Apoptosis. 2014 May;19(5):871-82. doi: 10.1007/s10495-014-0969-0. PubMed PMID: 24563182.

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Keywords

Clitocine 105798-74-1 Apoptosis BCL inhibitor inhibit

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