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CeMMEC1

Catalog No. T4345 CAS 440662-09-9

Synonyms: CeMMEC1 HCl

CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM).

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CeMMEC1, CAS 440662-09-9

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM).
Targets&IC50	TAF1:0.9 μM
In vitro	CeMMEC1 exhibits high affinity activity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1.
Kinase Assay	TAF1 binding assays are conducted using the EPIgeneous Binding Domain kit B. Binding is determined by the displacement of an acetylated biotin peptide from a GST-tagged TAF1 protein using HTRF with a Eu3+-conjugated GST antibody donor and streptavidin-conjugated acceptor. Compounds (CeMMEC1) are dispensed into assay plates, ProxiPlate-384 Plus using an Echo 525 Liquid Handler. Binding assays are conducted in a final volume of 20 μL with 5 nM TAF1-GST, 50 nM peptide (SGRGK (ac)GGK (ac)GLGK (ac)GGAK (ac)RHRK (biotin)-acid), 6.25 nM Streptavidin-XL665, 1:200 Anti-GST-Eu3+ cryptate and 0.1% DMSO. Assay reagents are dispensed into plates using a Multidrop combi and incubated at room temperature for 3 h. Fluorescence is measured using a PHERAstar microplate reader using the HTRF module with dual emission protocol (A = excitation of 320 nm, emmission of 665 nm, and B = excitation of 320 nm, emission of 620 nm). Raw data are processed to give an HTRF ratio (channel A/B × 10,000), which is used to generate IC50 curves
Cell Research	REDS3 cells were treated with CeMMEC1(10 μM) incubating for 24 hours.

Synonyms	CeMMEC1 HCl
Molecular Weight	336.347
Formula	C19H16N2O4
CAS No.	440662-09-9

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 21 mg/mL(62.4 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Sdelci S,etal. Mapping the chemical chromatin reactivation landscape identifies BRD4-TAF1 cross-talk.Nat Chem Biol. 2016 Jul;12(7):504-10.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Inhibitor Library Anti-Metabolism Disease Compound Library PPI Inhibitor Library Chromatin Modification Compound Library Epigenetics Compound Library Target-Focused Phenotypic Screening Library Anti-Aging Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

CeMMEC1 440662-09-9 DNA损伤和修复表观遗传代谢细胞周期 Carboxypeptidase(CPG2) DNA/RNA Synthesis Epigenetic Reader Domain CeMMEC-1 CeMMEC1 HCl Inhibitor inhibit CeMMEC 1 inhibitor

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