Powder: -20°C for 3 years
In solvent: -80°C for 2 years
CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM).
Description | CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM). |
Targets&IC50 | TAF1:0.9 μM |
In vitro | CeMMEC1 exhibits high affinity activity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1. |
Kinase Assay | TAF1 binding assays are conducted using the EPIgeneous Binding Domain kit B. Binding is determined by the displacement of an acetylated biotin peptide from a GST-tagged TAF1 protein using HTRF with a Eu3+-conjugated GST antibody donor and streptavidin-conjugated acceptor. Compounds (CeMMEC1) are dispensed into assay plates, ProxiPlate-384 Plus using an Echo 525 Liquid Handler. Binding assays are conducted in a final volume of 20 μL with 5 nM TAF1-GST, 50 nM peptide (SGRGK (ac)GGK (ac)GLGK (ac)GGAK (ac)RHRK (biotin)-acid), 6.25 nM Streptavidin-XL665, 1:200 Anti-GST-Eu3+ cryptate and 0.1% DMSO. Assay reagents are dispensed into plates using a Multidrop combi and incubated at room temperature for 3 h. Fluorescence is measured using a PHERAstar microplate reader using the HTRF module with dual emission protocol (A = excitation of 320 nm, emmission of 665 nm, and B = excitation of 320 nm, emission of 620 nm). Raw data are processed to give an HTRF ratio (channel A/B × 10,000), which is used to generate IC50 curves |
Cell Research | REDS3 cells were treated with CeMMEC1(10 μM) incubating for 24 hours. |
Synonyms | CeMMEC1 HCl |
Molecular Weight | 336.347 |
Formula | C19H16N2O4 |
CAS No. | 440662-09-9 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 21 mg/mL(62.4 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
CeMMEC1 440662-09-9 DNA损伤和修复 表观遗传 代谢 细胞周期 Carboxypeptidase(CPG2) DNA/RNA Synthesis Epigenetic Reader Domain CeMMEC-1 CeMMEC1 HCl Inhibitor inhibit CeMMEC 1 inhibitor