Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CK-636 (CK-0944636), an Arp2/3 complex inhibitor, can inhibit actin polymerization induced by the human (IC50: 4 μM), fission yeast (IC50: 24 μM) and bovine (IC50: 32 μM) Arp2/3 complex, respectively.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 30.00 | |
10 mg | In stock | $ 47.00 | |
25 mg | In stock | $ 83.00 | |
50 mg | In stock | $ 139.00 | |
100 mg | In stock | $ 198.00 | |
500 mg | In stock | $ 516.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 32.00 |
Description | CK-636 (CK-0944636), an Arp2/3 complex inhibitor, can inhibit actin polymerization induced by the human (IC50: 4 μM), fission yeast (IC50: 24 μM) and bovine (IC50: 32 μM) Arp2/3 complex, respectively. |
Targets&IC50 | Bovine Arp2/3 complex:32 μM, Fission yeast Arp2/3 complex:24 μM, Human Arp2/3 complex:4 μM |
In vivo | CK-636 slows down cell migration by inhibiting the formation of lamellipodia at the leading edge of migrating T-cells. In live cells, it suppresses actin polymerization mediated by the Arp2/3 complex. In infected SKOV3 cells, CK-636 reduces the formation of actin filaments. Moreover, a majority of T-cells treated with CK-636 exhibit a turning event upon encountering a zigzag-shaped interface during a letter-shaped test experiment. |
Kinase Assay | HDAC inhibition assays: HDAC inhibition assays are performed by Reaction Biology Corp. using isolated human, recombinant full2length HDAC1 and -6 from a baculovirus expression system in Sf9 cells. An acetylated fluorogenic peptide,RHKKAc, derived from residues 379-382 of p53 is used as substrate. The reaction buffer is made up of 50 mM Tris-HCl pH 8.0, 127 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA, and a final concentration of 1% DMSO. Compounds are delivered in DMSO and delivered to enzyme mixture with preincubation of 5-10 min followed by substrate addition and incubation for 2 h at 30°C. Trichostatin A and developer are added to quench the reaction and generate fluorescence, respectively. Dose-response curves are generated starting at 30 μM compound with three-fold serial dilutions to generate a 10-dose plot. IC50 values are then generated from the resulting plots, and the values expressed are the average of duplicate trials ± standard deviation. |
Synonyms | CK-0944636, CK 636 |
Molecular Weight | 284.38 |
Formula | C16H16N2OS |
CAS No. | 442632-72-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 47 mg/mL (165.3 mM)
DMSO: 53 mg/mL (186.4 mM)
You can also refer to dose conversion for different animals. More
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CK-636 442632-72-6 Cytoskeletal Signaling Microtubule Associated CK 0944636 Inhibitor Actin-related protein 2/3 complex CK636 CK-0944636 CK0944636 Arp2/3 Complex inhibit CK 636 inhibitor