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CK-636

Catalog No. T1820   CAS 442632-72-6
Synonyms: CK-0944636, CK 636

CK-636 (CK-0944636), an Arp2/3 complex inhibitor, can inhibit actin polymerization induced by the human (IC50: 4 μM), fission yeast (IC50: 24 μM) and bovine (IC50: 32 μM) Arp2/3 complex, respectively.

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CK-636 Chemical Structure
CK-636, CAS 442632-72-6
Pack Size Availability Price/USD Quantity
5 mg In stock $ 30.00
10 mg In stock $ 47.00
25 mg In stock $ 83.00
50 mg In stock $ 139.00
100 mg In stock $ 198.00
500 mg In stock $ 516.00
1 mL * 10 mM (in DMSO) In stock $ 32.00
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Purity: 98.88%
Purity: 98.86%
Purity: 98.52%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description CK-636 (CK-0944636), an Arp2/3 complex inhibitor, can inhibit actin polymerization induced by the human (IC50: 4 μM), fission yeast (IC50: 24 μM) and bovine (IC50: 32 μM) Arp2/3 complex, respectively.
Targets&IC50 Bovine Arp2/3 complex:32 μM, Fission yeast Arp2/3 complex:24 μM, Human Arp2/3 complex:4 μM
In vivo CK-636 slows down cell migration by inhibiting the formation of lamellipodia at the leading edge of migrating T-cells. In live cells, it suppresses actin polymerization mediated by the Arp2/3 complex. In infected SKOV3 cells, CK-636 reduces the formation of actin filaments. Moreover, a majority of T-cells treated with CK-636 exhibit a turning event upon encountering a zigzag-shaped interface during a letter-shaped test experiment.
Kinase Assay HDAC inhibition assays: HDAC inhibition assays are performed by Reaction Biology Corp. using isolated human, recombinant full2length HDAC1 and -6 from a baculovirus expression system in Sf9 cells. An acetylated fluorogenic peptide,RHKKAc, derived from residues 379-382 of p53 is used as substrate. The reaction buffer is made up of 50 mM Tris-HCl pH 8.0, 127 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA, and a final concentration of 1% DMSO. Compounds are delivered in DMSO and delivered to enzyme mixture with preincubation of 5-10 min followed by substrate addition and incubation for 2 h at 30°C. Trichostatin A and developer are added to quench the reaction and generate fluorescence, respectively. Dose-response curves are generated starting at 30 μM compound with three-fold serial dilutions to generate a 10-dose plot. IC50 values are then generated from the resulting plots, and the values expressed are the average of duplicate trials ± standard deviation.
Synonyms CK-0944636, CK 636
Molecular Weight 284.38
Formula C16H16N2OS
CAS No. 442632-72-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 47 mg/mL (165.3 mM)

DMSO: 53 mg/mL (186.4 mM)

TargetMolReferences and Literature

1. Nolen BJ, et al. Nature. 2009 , 460(7258), 1031-1034. 2. Kwon KW, et al. PLoS One. 2013, 8(9), e73960.

TargetMolCitations

1. Zhu X, Zhao Y, Liu Y, et al.Macrophages release IL11-containing filopodial tip vesicles and contribute to renal interstitial inflammation.Cell Communication and Signaling.2023, 21(1): 1-17.

Related compound libraries

This product is contained In the following compound libraries:
Microtubule-Targeted Compound Library Bioactive Compound Library Cytoskeletal Signaling Pathway Compound Library Immunology/Inflammation Compound Library Inhibitor Library Bioactive Compounds Library Max NO PAINS Compound Library

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Keywords

CK-636 442632-72-6 Cytoskeletal Signaling Microtubule Associated CK 0944636 Inhibitor Actin-related protein 2/3 complex CK636 CK-0944636 CK0944636 Arp2/3 Complex inhibit CK 636 inhibitor

 

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