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Bafilomycin A1

Catalog No. T6740 CAS 88899-55-2

Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways. Bafilomycin A1 is a vacuolar H+-ATPase inhibitor (IC50: 0.44 nM). It is also found to inhibit autophagy while induces apoptosis.Bafilomycin A1 triggers proliferative potential of senescent cancer cells in vitro and in NOD/SCID mice.

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Bafilomycin A1, CAS 88899-55-2

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Purity: 98%

Biological Description

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Storage & Solubility Information

Description	Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways. Bafilomycin A1 is a vacuolar H+-ATPase inhibitor (IC50: 0.44 nM). It is also found to inhibit autophagy while induces apoptosis.Bafilomycin A1 triggers proliferative potential of senescent cancer cells in vitro and in NOD/SCID mice.
In vitro	Bafilomycin A1 at a concentration of 1 nM effectively and specifically kills pediatric B-cell acute lymphoblastic leukemia cells. Bafilomycin A1 induces the binding of Beclin 1 to Bcl-2, which further inhibits autophagy and promotes apoptotic cell death[1]. The growth of the BEL-7402 hepatocellular carcinoma and HO-8910 ovarian cancer cell lines are retarded and the metastatic potential is inhibited by Bafilomycin A1. Transmission electron microscopy and assays of capsase-3 and -9 suggest that Bafilomycin A1 induces apoptosis[2]. Bafilomycin A1 inhibits the growth of a variety of cultured cells dose-dependently, including golden hamster embryo, NIH-3T3 fibroblasts, PC12 and HeLa cells. The IC50 of Bafilomycin A1 for inhibition of cell growth ranges from 10 to 50 nM[3].
In vivo	Chronic treatment with Bafilomycin A1 at the dose of 1 mg/kg significantly inhibits the tumor growth compared with controls, after 21 days[4].

Molecular Weight	622.84
Formula	C35H58O9
CAS No.	88899-55-2

Storage

store at low temperature

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 99 mg/mL(158.95 mM)

H2O: 0.1 mg/mL (0.16 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. al-Fifi ZI, et al. Insect BiochemMol Biol, 1998, 28(4), 201-211. 2. Du L, Wu Y, Han X, et al. NICE‑3‑knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway[J]. Experimental and Therapeutic Medicine . 2021, 21(6): 1-8.

Citations

1. Zhang Y, Ding Y, Li M, et al. MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B. Acta Pharmaceutica Sinica B. 2021 2. Du L, Wu Y, Han X, et al. NICE-3-knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway. Experimental and Therapeutic Medicine. 2021, 21(6): 1-8 3. Wang J, Su Q, Wu Q, et al. Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells. Archives of Pharmacal Research. 2021: 1-12. 4. Su Q, Wu Q, Chen K, et al. Induction of Estrogen Receptor β-mediated Autophagy Sensitizes Breast Cancer Cells to TAD1822-7, a Novel Biphenyl Urea Taspine Derivative. Molecular Biology Reports. 2021

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

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