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Bafilomycin A1

Catalog No. T6740   CAS 88899-55-2

Bafilomycin A1 belongs to the macrolide class of antibiotics and is a V-ATPase (IC50=0.44 nM) that is specific and reversible. Bafilomycin A1 is an inhibitor of the late phase of autophagy, blocking the fusion of autophagosomes with lysosomes. Bafilomycin A1 also induces apoptosis.

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Bafilomycin A1 Chemical Structure
Bafilomycin A1, CAS 88899-55-2
Pack Size Availability Price/USD Quantity
1 mg In stock $ 287.00
5 mg In stock $ 699.00
160 μL * 1 mM (in DMSO) In stock $ 98.00
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Purity: 98.88%
Purity: 98.88%
Purity: 96.59%
Purity: 95.87%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Bafilomycin A1 belongs to the macrolide class of antibiotics and is a V-ATPase (IC50=0.44 nM) that is specific and reversible. Bafilomycin A1 is an inhibitor of the late phase of autophagy, blocking the fusion of autophagosomes with lysosomes. Bafilomycin A1 also induces apoptosis.
In vitro METHODS: Human pancreatic cancer cells Capan-1 were treated with Bafilomycin A1 (1-100 nM) for 72 h. Cell growth inhibition was detected using MTT.
RESULTS: Bafilomycin A1 dose-dependently inhibited the growth of Capan-1 cells with an IC50 of 5 nM. [1]
METHODS: Human osteosarcoma cells MG63 were treated with Bafilomycin A1 (1 μmol/L) for 6-24 h. The expression levels of target proteins were detected by Western Blot.
RESULTS: Bafilomycin A1 induced a significant increase in the levels of apoptosis-related proteins p53 and Beclin 1, a significant decrease in the expression of autophagy-related proteins p62 and LC3-I, and an increase in LC3-II. [2]
METHODS: Human hepatocellular carcinoma cells BEL7402 and HepG2 were incubated with Bafilomycin A1 (5 nM) for 24 h, and the cell cycle was examined by Flow Cytometry.
RESULTS: Bafilomycin A1 increased the percentage of BEL7402 and HepG2 cells in the G1 phase of the cell cycle, while cells in the G2/M phase decreased, indicating G1 phase arrest. [3]
In vivo METHODS: To assay antitumor activity in vivo, Bafilomycin A1 (0.1-1 mg/kg) was intraperitoneally injected once daily for three days into NOD-SCID mice harboring the human acute lymphoblastic leukemia tumor B-ALL.
RESULTS: Bafilomycin A1 prolonged the survival and improved the pathology of xenograft mice by targeting leukemia cells. [4]
METHODS: To investigate the role of autophagy in chronic wound healing, Bafilomycin A1 (1 mg/kg) was administered as a single intraperitoneal injection to diabetic db/db mice.
RESULTS: Bafilomycin A1 treatment significantly accelerated wound healing in db/db mice and exerted a favorable healing effect.Bafilomycin A1 may accelerate the healing of diabetic chronic refractory wounds by promoting cell proliferation, collagen production, and modulating inflammatory homeostasis. [5]
Molecular Weight 622.83
Formula C35H58O9
CAS No. 88899-55-2

Storage

store at low temperature,keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 99 mg/mL(158.95 mM)

H2O: 0.1 mg/mL (0.16 mM)

TargetMolReferences and Literature

1. Ohta T, et al. Bafilomycin A1 induces apoptosis in the human pancreatic cancer cell line Capan-J Pathol. 1998 Jul;185(3):324-30. 2. Xie Z, et al. Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells. Mol Med Rep. 2014 Aug;10(2):1103-7. 3. Yan Y, et al. Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways. Sci Rep. 2016 Nov 15;6:37052. 4. Yuan N, et al. Bafilomycin A1 targets both autophagy and apoptosis pathways in pediatric B-cell acute lymphoblastic leukemia. Haematologica. 2015 Mar;100(3):345-56. 5. Wang F, et al. Bafilomycin A1 Accelerates Chronic Refractory Wound Healing in db/db Mice. Biomed Res Int. 2020 Jul 2;2020:6265701.

TargetMolCitations

1. Zhang Y, Ding Y, Li M, et al. MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B. Acta Pharmaceutica Sinica B. 2021 2. Du L, Wu Y, Han X, et al. NICE-3-knockdown induces cell cycle arrest and autophagy in lung adenocarcinoma cells via the AKT/mTORC1 signaling pathway. Experimental and Therapeutic Medicine. 2021, 21(6): 1-8 3. Wang J, Su Q, Wu Q, et al. Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells. Archives of Pharmacal Research. 2021: 1-12. 4. Su Q, Wu Q, Chen K, et al. Induction of Estrogen Receptor β-mediated Autophagy Sensitizes Breast Cancer Cells to TAD1822-7, a Novel Biphenyl Urea Taspine Derivative. Molecular Biology Reports. 2021 5. Wang J, Yan J T, Zeng S T, et al.Revealing Mitochondrion–Lysosome Dynamic Interactions and pH Variations in Live Cells with a pH-Sensitive Fluorescent Probe.Analytical Chemistry.2023 6. Gao C, Shi Q, Pan X, et al.Neuromuscular organoids model spinal neuromuscular pathologies in C9orf72 amyotrophic lateral sclerosis.Cell Reports.2024, 43(3). 7. Bao Q, Wang A, Hong W, et al.The c-Abl-RACK1-FAK signaling axis promotes renal fibrosis in mice through regulating fibroblast-myofibroblast transition.Cell Communication and Signaling.2024, 22(1): 247.

Related compound libraries

This product is contained In the following compound libraries:
Microbial Natural Product Library Natural Product Library for HTS Anti-Cancer Compound Library NO PAINS Compound Library Rare Natural Product Library Anti-Infection Compound Library

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Keywords

Bafilomycin A1 88899-55-2 Apoptosis Autophagy Membrane transporter/Ion channel Microbiology/Virology Proton pump Antibiotic Antibacterial Proton Pump Bafilomycin A 1 Bafilomycin A-1 BafA1 BafA 1 inhibit BafA-1 Inhibitor Bacterial inhibitor

 

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