Powder: -20°C for 3 years | In solvent: -80°C for 1 year
AGI-6780 is a potent and selective inhibitor of IDH2 R140Q mutant.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 31.00 | |
2 mg | In stock | $ 44.00 | |
5 mg | In stock | $ 72.00 | |
10 mg | In stock | $ 122.00 | |
25 mg | In stock | $ 232.00 | |
50 mg | In stock | $ 413.00 | |
100 mg | In stock | $ 618.00 | |
500 mg | In stock | $ 1,330.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 77.00 |
Description | AGI-6780 is a potent and selective inhibitor of IDH2 R140Q mutant. |
Targets&IC50 | IDH2 R140Q mutant:23 nM |
Kinase Assay | AGI-6780 is prepared as 10 mM stock in DMSO and diluted to 50X final concentration in DMSO, for a 50 μL reaction mixture. IDH enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate is measured using a NADPH depletion assay. In the assay the remaining cofactor is measured at the end of the reaction with the addition of a catalytic excess of diaphorase and resazurin, to generate a fluorescent signal in proportion to the amount of NADPH remaining. IDH enzyme activity in the direction of isocitrate to alpha-ketoglutarate conversion is measured by direct coupling of the NADPH production to conversion of resazurin to resorufin by diaphorase. In both cases, resorufin is measured fluorometrically at Ex544 Em590[1]. |
Cell Research | AGI-6780 is dissolved in DMSO and stored, and then diluted with appropriate media before use[1]. Cells are sorted from fresh or frozen bone marrow aspirates and blood samples after labelling with PE-CD34, APC-CD38, PE-CD14, FITC-CD3 (clone HIT3a) and PECy7-CD19 (clone SJ25C1) antibodies using a MoFlow cell sorter. Unfractionated nucleated blood or bone marrow cells are plated in Methocult H4434 methylcellulose medium at 104 cells/dish, in duplicate dishes per condition. AGI-6780 (5 mM) is directly added to the medium. Dishes are incubated in a humidified incubator at 37°C and colonies containing at least 30 cells are counted after 13 days[1]. |
Molecular Weight | 481.51 |
Formula | C21H18F3N3O3S2 |
CAS No. | 1432660-47-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 89 mg/mL (184.8 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 89 mg/mL (184.8 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
AGI-6780 1432660-47-3 Metabolism Dehydrogenase Isocitrate Dehydrogenase (IDH) inhibit AGI6780 Inhibitor AGI 6780 inhibitor