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7 Beta-Hydroxycholesterol

Name: 7 Beta-Hydroxycholesterol
Brand: TargetMol
SKU: TN1338
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TN1338Cas No. 566-27-8

Alias 7β-Hydroxycholesterol

7 Beta-Hydroxycholesterol (7β-Hydroxycholesterol) is an oxysterol contained in animal-derived foods. It is an oxidative stress inducer and a candidate marker for assessing cardiovascular risk factors in obesity. It can induce oxidative stress and inflammation and promote atherosclerosis.

7 Beta-Hydroxycholesterol

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Purity: 99.24%

Catalog No. TN1338Alias 7β-HydroxycholesterolCas No. 566-27-8

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$48	-	In Stock
5 mg	$147	-	In Stock
10 mg	$239	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.24%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	7 Beta-Hydroxycholesterol (7β-Hydroxycholesterol) is an oxysterol contained in animal-derived foods. It is an oxidative stress inducer and a candidate marker for assessing cardiovascular risk factors in obesity. It can induce oxidative stress and inflammation and promote atherosclerosis.
In vitro	b>METHODS: Human NCI-H460 lung cancer cells were treated with 7β-Hydroxycholesterol (10, 20 and 30 μM for 14 days) and subjected to a clonogenic survival assay. RESULTS: 7β-Hydroxycholesterol inhibited the clonogenic survival of NCI-H460 cells in a dose-dependent manner, with survival rates of 102%, 75%, 35% and 12% at concentrations of 5, 10, 20 and 30 μM, respectively. [1]
Synonyms	7β-Hydroxycholesterol

Chemical Properties

Molecular Weight	402.65
Formula	C₂₇H₄₆O₂
Cas No.	566-27-8
Smiles	C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=C[C@@H]3O)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@H](CCCC(C)C)C)[H])[H]
Relative Density.	1.03 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 80 mg/mL (198.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (8.2 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4835 mL	12.4177 mL	24.8355 mL	124.1773 mL
5 mM	0.4967 mL	2.4835 mL	4.9671 mL	24.8355 mL
10 mM	0.2484 mL	1.2418 mL	2.4835 mL	12.4177 mL
20 mM	0.1242 mL	0.6209 mL	1.2418 mL	6.2089 mL
50 mM	0.0497 mL	0.2484 mL	0.4967 mL	2.4835 mL
100 mM	0.0248 mL	0.1242 mL	0.2484 mL	1.2418 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc