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7α,25-Dihydroxycholesterol

Catalog No. T14063   CAS 64907-22-8
Synonyms: 7α,25-OHC

7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells[1][2], and it is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM).

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7α,25-Dihydroxycholesterol Chemical Structure
7α,25-Dihydroxycholesterol, CAS 64907-22-8
Pack Size Availability Price/USD Quantity
25 mg 6-8 weeks $ 992.00
50 mg 6-8 weeks $ 1,289.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description 7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells[1][2], and it is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM).
In vitro 7α, 25-dihydroxycholesterol (7α,25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM in vitro. However, it had no effect on EBI2-deficient cells[1].
In vivo In vivo, EBI2-deficient B cells or normal B cells desensitized by 7α,25-Dihydroxycholesterol (1 μM; 1.5 hours) pre-treatment shows reduced homing to follicular areas of the spleen[1].
Synonyms 7α,25-OHC
Molecular Weight 418.65
Formula C27H46O3
CAS No. 64907-22-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 1 mg/mL (2.39 mM), sonification is recommended.

TargetMolReferences and Literature

1. Liu C, et al. Oxysterols direct B-cell migration through EBI2. Nature. 2011 Jul 27;475(7357):519-23. 2. Hannedouche S, et al. Oxysterols direct immune cell migration via EBI2. Nature. 2011 Jul 27;475(7357):524-7.

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Keywords

7α,25-Dihydroxycholesterol 64907-22-8 GPCR/G Protein Metabolism EBI2/GPR183 Endogenous Metabolite endogenous response 7a,25-OHC oxysterols 7a,25-Dihydroxycholesterol 7alpha,25-Dihydroxycholesterol GPCR ligand 7α,25 Dihydroxycholesterol receptor Inhibitor immune 7alpha,25-OHC migration 7α,25Dihydroxycholesterol cells 7α,25-OHC inhibit inhibitor

 

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