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3,3'-Diindolylmethane

Name: 3,3'-Diindolylmethane
Brand: TargetMol
SKU: T2991
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2991Cas No. 1968-05-4

Alias HB 236, DIM, Arundine

3,3'-Diindolylmethane (DIM), a small molecule compound, is a proposed Y preventive agent.

3,3'-Diindolylmethane

🥰Excellent

Purity: 99.09%

Catalog No. T2991Alias HB 236, DIM, ArundineCas No. 1968-05-4

3,3'-Diindolylmethane (DIM), a small molecule compound, is a proposed Y preventive agent.

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$39	In Stock	In Stock
100 mg	$53	In Stock	In Stock
200 mg	$62	In Stock	In Stock
500 mg	$81	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$57	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.09%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	3,3'-Diindolylmethane (DIM), a small molecule compound, is a proposed Y preventive agent.
In vitro	3,3'-Diindolylmethane is a potent radioprotector and mitigator that functions by stimulating an ATM-driven DDR-like response and NF-κB survival signaling. 3,3'-Diindolylmethane can inhibit invasion, angiogenesis, and proliferation and induce apoptosis in tumor cells by modulating signaling pathways involving AKT, NF-κB, and FOXO3. It can also inhibit estrogen-inducible gene expression and cause an endoplasmic reticulum stress response. 3,3'-Diindolylmethane alters estrogen metabolism by shifting metabolism from carcinogenic 16α-hydroxy to inert 2-hydroxy derivatives, and it antagonizes estrogen and androgen receptor activity[1].
In vivo	Preliminary studies show 3,3'-Diindolylmethane is most effective against total body irradiation (TBI) when given in multiple once-daily doses. Treatment of 3,3'-Diindolylmethane has radioprotection and mitigation properties in vivo. 3,3'-Diindolylmethane Activates ATM in Normal Tissues. 3,3'-Diindolylmethane can be given to mice (250 mg/kg) by oral gavage, with no acute toxicity and excellent bioavailability[1].
Cell Research	The 184A1 and Hs578Bst cells are pretreated with DIM (0.3 μM) or vehicle for 24 h, irradiated by using different doses of 137Cs γ rays, harvested, plated at different densities, incubated for 14 d, and counted for colony formation.(Only for Reference)
Synonyms	HB 236, DIM, Arundine

Chemical Properties

Molecular Weight	246.31
Formula	C₁₇H₁₄N₂
Cas No.	1968-05-4
Smiles	C(C1=CNC2=C1C=CC=C2)C1=CNC2=C1C=CC=C2
Relative Density.	1.271 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 18 mg/mL (73.08 mM), Sonication is recommended.

DMSO: 50 mg/mL (203 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (8.12 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.0599 mL	20.2996 mL	40.5992 mL	202.9962 mL
5 mM	0.8120 mL	4.0599 mL	8.1198 mL	40.5992 mL
10 mM	0.4060 mL	2.0300 mL	4.0599 mL	20.2996 mL
20 mM	0.2030 mL	1.0150 mL	2.0300 mL	10.1498 mL
50 mM	0.0812 mL	0.4060 mL	0.8120 mL	4.0599 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0406 mL	0.2030 mL	0.4060 mL	2.0300 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc