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25-Hydroxycholesterol

Name: 25-Hydroxycholesterol
Brand: TargetMol
SKU: T4717
Price: 45 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4717Cas No. 2140-46-7

25-Hydroxycholesterol is a steroid derivative that suppresses the cleavage of sterol regulatory element binding proteins (SREBPs). It inhibits HMG-CoA reductase and so it also plays a significant role in the in vivo regulation of cholesterol biosynthesis after an acute dietary cholesterol challenge. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

25-Hydroxycholesterol

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Purity: 99.89%

Catalog No. T4717Cas No. 2140-46-7

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$45	In Stock	In Stock
10 mg	$61	In Stock	In Stock
25 mg	$93	In Stock	In Stock
50 mg	$142	In Stock	In Stock
100 mg	$223	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.89%

Color:White

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COA HNMR LCMS

Product Introduction

25-Hydroxycholesterol AI Summary

25-Hydroxycholesterol exhibits a multifaceted pharmacological profile with significant bioactivities across various assays and targets. It activates the human nuclear oxysterol receptor liver X receptor-alpha (LXR-alpha) with an EC50 of 1160.0 nM and a relative efficacy of 0.35. As an HMG-CoA reductase inhibitor, it shows a 70.0% inhibition of transcription in HepG2 cells and an IC50 of 300.0 nM, suggesting potential for managing cholesterol metabolism. It also demonstrates agonist activity at the hedgehog receptor (Hh) with an EC50 of 1000.0 nM and induces RORgamma LBD coactivator recruitment with an EC50 of 20.0 nM. 25-Hydroxycholesterol effectively inhibits SREBP2 activation in CHO-K1 cells and shows cytotoxicity against human HepG2 cells (>70.0% at 48 hours as assessed by MTT assay). Additionally, it exhibits binding affinity to NPC1L1 mutants expressed in HEK293 cells, shown by fluorescence microscopy with an EC50 of 2300.0 nM. Despite its extensive bioactivities, 25-Hydroxycholesterol displays minimal cytotoxic and antitubercular activities against Vero cells and Mycobacterium tuberculosis H37Rv. It also has positive effects on reducing alpha-crystallin B R120G mutant protein aggregation in HeLa and HLE-B3 cells. Other notable activities include its impact on the SARS-CoV-2 3CL-Pro protease and an antiviral effect against SARS-CoV-2 induced cytotoxicity in Vero-6 cells. It also has a binding affinity to human integrin alphavbeta3 (Kd value of 10.9 nM) and degrades human HMGCR-dCat-ELuc in HEK293 cells with an EC50 of 630.0 nM. 25-Hydroxycholesterol displays a broad yet potent spectrum spanning several targets and pathways, making it a promising candidate for various therapeutic applications..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

Chemical Properties

Molecular Weight	402.65
Formula	C₂₇H₄₆O₂
Cas No.	2140-46-7
Smiles	C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
Relative Density.	1.03g/cm3

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 20 mg/mL (49.67 mM), Sonication is recommended.

Chloroform: 50 mg/mL (124.18 mM), Sonication is recommended.

Solution Preparation Table

Ethanol/Chloroform

	1mg	5mg	10mg	50mg
1 mM	2.4835 mL	12.4177 mL	24.8355 mL	124.1773 mL
5 mM	0.4967 mL	2.4835 mL	4.9671 mL	24.8355 mL
10 mM	0.2484 mL	1.2418 mL	2.4835 mL	12.4177 mL
20 mM	0.1242 mL	0.6209 mL	1.2418 mL	6.2089 mL

Chloroform

	1mg	5mg	10mg	50mg
50 mM	0.0497 mL	0.2484 mL	0.4967 mL	2.4835 mL
100 mM	0.0248 mL	0.1242 mL	0.2484 mL	1.2418 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc