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20(S)-Hydroxycholesterol

Catalog No. T10085 CAS 516-72-3

Synonyms: 20α-Hydroxycholesterol

20(S)-Hydroxycholesterol is an allosteric activator of the oncoprotein smoothened (Smo).

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20(S)-Hydroxycholesterol, CAS 516-72-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	20(S)-Hydroxycholesterol is an allosteric activator of the oncoprotein smoothened (Smo).
In vitro	20(S)-Hydroxycholesterol activates the hedgehog signaling pathway with an EC50 of 3 μM in NIH3T3 cells[2].

Synonyms	20α-Hydroxycholesterol
Molecular Weight	402.65
Formula	C27H46O2
CAS No.	516-72-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 27mg/mL (67.1mM), ultrasonic and warming and heat to 60°C

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Nachtergaele S, et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol. 2012 Jan 8;8(2):211-20. 2. Nedelcu D, et al, Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling. Nat Chem Biol. 2013 Sep;9(9):557-64.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

20(S)-Hydroxycholesterol 516-72-3 其他 Others 20α-Hydroxycholesterol Inhibitor inhibitor inhibit

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