SOST Protein, Human, Recombinant (His)

Catalog No. TMPY-00925

SOST Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1.

SOST Protein, Human, Recombinant (His)

Catalog No. TMPY-00925

SOST Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1.

Pack Size	Price	Availability
50 μg	$197	In Stock
100 μg	$346	7-10 days
200 μg	$612	7-10 days
500 μg	$1,300	7-10 days

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Product Information

Biological Activity	Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells. The ED50 for this effect is typically 0.2-1 μg/mL.
Description	SOST Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1.
Species	Human
Expression System	HEK293 Cells
Tag	N-His
Accession Number	Q9BQB4-1
Synonyms	VBCH,SOST1,sclerostin,DAND6,CDD
Construction	A DNA sequence encoding the mature form of human SOST (NP_079513.1) (Gln24-Tyr213) was fused with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	22.5 kDa (predicted); 29.9 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation.