IL-5 Protein, Mouse, Recombinant (His)

Name: IL-5 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02554
Rating: 4.5 (1 reviews)

(Synonyms: interleukin 5, Il-5) Copy Product Info

Synonyms: interleukin 5, Il-5

Catalog No. TMPY-02554 Copy Product Info

Purity: 99.2%

IL-5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P04401.

Pack Size	Price	EUR Stock	Global Stock
5 µg	38 €	7-10 days	7-10 days
10 µg	59 €	7-10 days	7-10 days
20 µg	95 €	In Stock	In Stock
50 µg	199 €	7-10 days	7-10 days
100 µg	357 €	7-10 days	7-10 days
200 µg	630 €	7-10 days	7-10 days
500 µg	1.332 €	7-10 days	7-10 days

For In stock only · Estimated delivery: EUR Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.2%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
Description	IL-5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P04401.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	P04401
Construction	A DNA sequence encoding the mouse IL5 (P04401) (Met 1-Gly 133) was expressed，with a C-terminal polyhistidine tag. Predicted N terminal: Met 21
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.0, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	interleukin 5, Il-5
Research Background	Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.

Chemical Properties

Molecular Weight

14.5 kDa (predicted); 18 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.