IL-5 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02554

IL-5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P04401.

IL-5 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-02554

IL-5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P04401.

Pack Size	Price	Availability
20 μg	$106	In Stock
100 μg	$397	7-10 days
200 μg	$700	7-10 days
500 μg	$1,480	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/mL.
Description	IL-5 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 14.5 kDa and the accession number is P04401.
Species	Mouse
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	P04401
Synonyms	interleukin 5,Il-5
Construction	A DNA sequence encoding the mouse IL5 (P04401) (Met 1-Gly 133) was expressed，with a C-terminal polyhistidine tag. Predicted N terminal: Met 21
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	14.5 kDa (predicted); 18 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.0, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.