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Synonyms: P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $44 | 7-10 days | 7-10 days | |
| 10 μg | $68 | 7-10 days | 7-10 days | |
| 20 μg | $108 | 7-10 days | 7-10 days | |
| 50 μg | $215 | 7-10 days | 7-10 days | |
| 100 μg | $413 | 7-10 days | 7-10 days | |
| 200 μg | $693 | 7-10 days | 7-10 days | |
| 500 μg | $1,670 | 7-10 days | 7-10 days | |
| 1 mg | $3,750 | 7-10 days | 7-10 days |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >40% cells will adhere after 1 hour of incubation at 37℃. |
| Description | ICAM-1/CD54 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 77.4 kDa and the accession number is A0A384MEK5. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | His, hFc |
| Accession Number | P05362 |
| Construction | A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gln 28 |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2 |
| Research Background |
| Molecular Weight | 77.4 kDa (predicted); 100-110 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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