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ICAM-1/CD54 Protein, Human, Recombinant (His & hFc)

(Synonyms: P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2) Copy Product Info

Synonyms: P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2

Catalog No. TMPY-00825 Copy Product Info
ICAM-1/CD54 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 77.4 kDa and the accession number is A0A384MEK5.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$447-10 days7-10 days
10 μg$687-10 days7-10 days
20 μg$1087-10 days7-10 days
50 μg$2157-10 days7-10 days
100 μg$4137-10 days7-10 days
200 μg$6937-10 days7-10 days
500 μg$1,6707-10 days7-10 days
1 mg$3,7507-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >40% cells will adhere after 1 hour of incubation at 37℃.
Description
ICAM-1/CD54 Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 77.4 kDa and the accession number is A0A384MEK5.
Species
Human
Expression System
HEK293 Cells
TagHis, hFc
Accession NumberP05362
ConstructionA DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gln 28
Protein Purity
> 95 % as determined by SDS-PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsP3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Research Background
Chemical Properties
Molecular Weight77.4 kDa (predicted); 100-110 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: ICAM-1/CD54 Protein, Human, Recombinant (His & hFc) chemical structure | ICAM-1/CD54 Protein, Human, Recombinant (His & hFc) molecular weight