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HRO761

Name: HRO761
Brand: TargetMol
SKU: T72107
Price: 55 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Werner syndrome RecQ helicase-IN-1, HRO-761) Copy Product Info

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Synonyms: Werner syndrome RecQ helicase-IN-1, HRO-761

Catalog No. T72107 Copy Product Info

Purity: 99.62%

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HRO761 (Werner syndrome RecQ helicase-IN-1, Example 42) is a small molecule inhibitor, a potent WRN inhibitor with high efficacy, and can be used for research on cancers such as colon cancer and gastric cancer.

Cas No. 2869954-34-5

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Pack Size	Price	USA Stock	Global Stock
1 mg	$55	In Stock	In Stock
5 mg	$127	In Stock	In Stock
10 mg	$186	In Stock	In Stock
25 mg	$312	In Stock	In Stock
50 mg	$528	In Stock	In Stock
100 mg	$787	-	In Stock
1 mL x 10 mM (in DMSO)	$239	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.62%

Appearance:Solid

Color:White to Yellow

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	HRO761 (Werner syndrome RecQ helicase-IN-1, Example 42) is a small molecule inhibitor, a potent WRN inhibitor with high efficacy, and can be used for research on cancers such as colon cancer and gastric cancer.
Targets & IC50	WRN ATPase:100 nM, SW48 cells:50 nM, DLD1 WRN-KO cells:> 10 μM
In vitro	Methods: HRO761 was administered to SW48 (MSI) and CAL33 (MSS) cells for 4 days. Results: CellTiter-Glo assay demonstrated selective inhibition of MSI cell proliferation with a GI50 of 40 nM, while showing no activity against MSS cells. Colony formation assays (10–14 days) confirmed GI50 ranges of 50–1000 nM in MSI cells. Western blot detected activation of DNA damage markers and WRN degradation. [1] Methods: An LC-MS/MS method was established for the determination of HRO761 concentration in rat plasma and applied to pharmacokinetic studies. Results: Following oral administration of HRO761 (5, 10, 20 mg/kg), rapid absorption was observed with time to peak concentration of 1.0–4.0 h, and absolute bioavailability ranged from 79.0% to 99.1%. [2]
In vivo	Methods: In the SW48 cell-derived xenograft tumor model (female nude mice), HRO761 was administered orally (20, 40, 60, 120 mg/kg, once daily, dissolved in 20% hydroxypropyl-β-cyclodextrin) for 92 consecutive days of treatment. Results: The 20 mg/kg dose achieved tumor stasis, while 60-120 mg/kg doses induced 75-90% tumor regression in a dose-dependent manner. [1]
Synonyms	Werner syndrome RecQ helicase-IN-1, HRO-761

Chemical Properties

Molecular Weight	702.08
Formula	C₃₁H₃₁ClF₃N₉O₅
Cas No.	2869954-34-5
Smiles	C(C(NC1=C(Cl)C=C(C(F)(F)F)C=C1)=O)N2C=3N(C(=O)C(=C2CC)N4CCN(C(=O)C=5C(O)=C(C)N=CN5)CC4)N=C(N3)C=6CCOCC6

Storage & Solubility Information

Storage

Store at low temperature,Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 99.7 mg/mL (142.01 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (4.7 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.4243 mL	7.1217 mL	14.2434 mL	71.2170 mL
5 mM	0.2849 mL	1.4243 mL	2.8487 mL	14.2434 mL
10 mM	0.1424 mL	0.7122 mL	1.4243 mL	7.1217 mL
20 mM	0.0712 mL	0.3561 mL	0.7122 mL	3.5608 mL
50 mM	0.0285 mL	0.1424 mL	0.2849 mL	1.4243 mL
100 mM	0.0142 mL	0.0712 mL	0.1424 mL	0.7122 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.