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UCF 101

Name: UCF 101
Brand: TargetMol
SKU: T21919
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T21919Cas No. 313649-08-0

UCF 101 is a specific inhibitor of HtrA2 and reduces apoptosis in PC12 cells.

UCF 101

🥰Excellent

Purity: 99.64%

Catalog No. T21919Cas No. 313649-08-0

UCF 101 is a specific inhibitor of HtrA2 and reduces apoptosis in PC12 cells.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$34	In Stock	In Stock
2 mg	$48	In Stock	In Stock
5 mg	$80	In Stock	In Stock
10 mg	$139	In Stock	In Stock
25 mg	$287	In Stock	In Stock
50 mg	$455	In Stock	In Stock
100 mg	$656	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$89	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.64%

Appearance:Solid

Color:Red

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	UCF 101 is a specific inhibitor of HtrA2 and reduces apoptosis in PC12 cells.
Targets&IC50	His-Omi:9.5 μM (IC50)
In vitro	PC12 cells treated with 60 μM 6-OHDA for 24 h had significantly decreased cell viability compared to that of controls. A low concentration (2.5 μM) of UCF 101 decreased the apoptosis rate of the PD cell model, but a high concentration (≥10 μM) increased the apoptosis rate, compared to that of controls. 6-OHDA upregulated the expression of HtrA2, α-syn, CHOP, Grp78 and active caspase-3 and reduced the levels of TH and XIAP[3].
In vivo	Administration of UCF 101 shortly before reperfusion reduced postischemic myocardial apoptosis in a dose-dependent fashion, with the maximal protective effect observed at a dose of 1.5 μmol/kg. This dose of UCF 101 (ie, 1.5 μmol/kg) was used in all subsequent experiments. To further establish that inhibition of Omi/HtrA2 protease activity led to significant inhibition of apoptosis, the effect of UCF 101 on DNA ladder formation, a hallmark of apoptotic cell death, was determined. 30 minutes of ischemia followed by 3 hours of reperfusion resulted in significant DNA fragmentation, which was significantly reduced by treatment with UCF 101 before reperfusion[2].UCF 101 reduced the level of ERS and apoptosis in vivo. The ratio of p-GSK3β (Tyr216 to Ser9) increased in PD rats[3].

Chemical Properties

Molecular Weight	495.51
Formula	C₂₇H₁₇N₃O₅S
Cas No.	313649-08-0
Smiles	[O-][N+](=O)c1ccccc1-c1ccc(C=C2C(=O)N(C(=S)N(C2=O)c2ccccc2)c2ccccc2)o1
Relative Density.	1.50 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 12.5 mg/mL (25.23 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0181 mL	10.0906 mL	20.1812 mL	100.9061 mL
5 mM	0.4036 mL	2.0181 mL	4.0362 mL	20.1812 mL
10 mM	0.2018 mL	1.0091 mL	2.0181 mL	10.0906 mL
20 mM	0.1009 mL	0.5045 mL	1.0091 mL	5.0453 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc